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EMBO Rep

Title:		Saccharomyces cerevisiae Rad16 mediates ultraviolet-dependent histone H3 acetylation required for efficient global genome nucleotide-excision repair
Author(s):		Teng Y; Liu H; Gill HW; Yu Y; Waters R; Reed SH;
Address:		"Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK"
Journal Title:		EMBO Rep
Year:		2008
Volume:		20071109
Issue:		1
Page Number:		97 - 102
DOI:		10.1038/sj.embor.7401112
ISSN/ISBN:		1469-221X (Print) 1469-3178 (Electronic) 1469-221X (Linking)
Abstract:		"In yeast, global genome nucleotide-excision repair (GG-NER) requires a protein complex containing Rad7 and Rad16. Rad16 is a member of the switch/sucrose nonfermentable superfamily, and it is presumed that chromatin remodelling is its primary function during repair. We show that RAD16 is required for ultraviolet-dependent hyperacetylation of histone H3 (Lys 9 and Lys 14) at the MFA2 promoter and throughout the genome. The yeast repressor complex Ssn6-Tup1 represses many genes including MFA2. TUP1 deletion results in constitutive hyperacetylation of histone H3, nucleosome disruption and derepression of gene transcription in Tup1-regulated genes. GG-NER in the MFA2 promoter proceeds more rapidly in tup1Delta alpha-cells compared with wild type, even when transcription is inhibited. We show that elevated histone H3 acetylation levels in the MFA2 promoter in tup1Delta alpha-cells result in Rad7- and Rad16-independent GG-NER, and that Rad16 mediates the ultraviolet-induced acetylation of histone H3, necessary for efficient GG-NER"
Keywords:		"Acetylation/radiation effects Adenosine Triphosphatases/*metabolism DNA Repair/*radiation effects DNA-Binding Proteins/metabolism Gene Deletion Gene Expression Regulation, Fungal/radiation effects Genes, Fungal Genome, Fungal/*genetics/radiation effects H;"
Notes:		"MedlineTeng, Yumin Liu, Hairong Gill, Hefin W Yu, Yachuan Waters, Raymond Reed, Simon H eng G0400113/MRC_/Medical Research Council/United Kingdom G9900118/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2007/11/17 EMBO Rep. 2008 Jan; 9(1):97-102. doi: 10.1038/sj.embor.7401112. Epub 2007 Nov 9"