« Previous Abstract"Effect of Gamma Irradiation on Fecundity, Sterility, and Female Sex Pheromone Production of Callosobruchus chinensis (Coleoptera: Bruchidae)"    Next AbstractGaseous and Particulate Emissions from Diesel Engines at Idle and under Load: Comparison of Biodiesel Blend and Ultralow Sulfur Diesel Fuels »

Microb Pathog

Title:		Characterization of essential oil from Ocimum gratissimum leaves: Antibacterial and mode of action against selected gastroenteritis pathogens
Author(s):		Chimnoi N; Reuk-Ngam N; Chuysinuan P; Khlaychan P; Khunnawutmanotham N; Chokchaichamnankit D; Thamniyom W; Klayraung S; Mahidol C; Techasakul S;
Address:		"Laboratory of Natural Products, Chulabhorn Research Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand. Laboratory of Organic Synthesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand. Laboratory of Biochemistry, Chulabhorn Research Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand. Office of Research, Chulabhorn Research Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand. Department of Biology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. Laboratory of Organic Synthesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand. Electronic address: supanna@cri.or.th"
Journal Title:		Microb Pathog
Year:		2018
Volume:		20180322
Issue:
Page Number:		290 - 300
DOI:		10.1016/j.micpath.2018.03.041
ISSN/ISBN:		1096-1208 (Electronic) 0882-4010 (Linking)
Abstract:		"Essential oil of fresh leaves of Ocimum gratissimum (OGEO) was water-steam distilled and analyzed by GC-MS. Thirty-seven compounds were identified, with eugenol (55.6%) as the major component followed by cis-ocimene (13.9%), gamma-muurolene (11.6%), (Z,E)-alpha-farnesene (5.6%), alpha-trans-bergamotene (4.1%), and beta-caryophyllene (2.7%). Antimicrobial activity of OGEO was tested against four gastroenteritis pathogens (Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Shigella flexneri). OGEO exhibited antibacterial effect, with MICs of 1-2?ª+mg?ª+ml(-1), against the tested species. OGEO also displayed rapid killing effect within 5?ª+s at four times of MIC against both E. coli and S. Typhimurium. Various assays were performed to investigate the mode of action of the oil. OGEO increased the permeability of microbial cell membrane as evidenced by LIVE/DEAD BacLight assay. Analyses of the release of absorbing materials at 260?ª+nm, protein leakage, SDS-PAGE, and SEM strongly suggested the disruptive action of the oil on the cytoplasmic membrane of the tested microorganisms. Results revealed that the antibacterial property of OGEO could be due to membrane disruption"
Keywords:		Anti-Bacterial Agents/*pharmacology Cell Membrane Permeability/drug effects Escherichia coli/drug effects Eugenol/chemistry Gas Chromatography-Mass Spectrometry Gastroenteritis/*microbiology Microbial Sensitivity Tests Microbial Viability Ocimum/*chemistr;
Notes:		"MedlineChimnoi, Nitirat Reuk-Ngam, Nanthawan Chuysinuan, Piyachat Khlaychan, Panita Khunnawutmanotham, Nisachon Chokchaichamnankit, Daranee Thamniyom, Wassapol Klayraung, Srikanjana Mahidol, Chulabhorn Techasakul, Supanna eng England 2018/03/27 Microb Pathog. 2018 May; 118:290-300. doi: 10.1016/j.micpath.2018.03.041. Epub 2018 Mar 22"