| Title: | Transcription and regulatory signals at the mating type locus in yeast |
| Author(s): | Siliciano PG; Tatchell K; |
| DOI: | 10.1016/0092-8674(84)90431-8 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "The mating type locus with two alleles (MATa and MAT alpha) determines cell type in yeast by activating and repressing sets of cell-type-specific genes. The two genes at MAT alpha, alpha 1 and alpha 2, are transcribed divergently from a central promoter region. Deletions in this intergenic region have been used to map DNA sequences involved in the transcription and regulation of the MAT alpha genes. A single promoter region, essential for transcription of both alpha 1 and alpha 2, is found in the region between alpha 1 and alpha 2. Deletions removing the alpha 1 or alpha 2 TATA box are still transcribed but the transcripts fail to initiate properly. A separate regulatory region is also found between alpha 1 and alpha 2. Deletions of this region lead to the constitutive expression of these genes. These regulatory mutants synthesize alpha 1 mRNA in diploids, but this is not sufficient to activate the alpha-specific genes" |
| Keywords: | "Base Sequence Chromosome Deletion DNA, Fungal/genetics Gene Expression Regulation *Genes, Fungal *Genes, Mating Type, Fungal Genes, Regulator Mating Factor Mutation Operon Peptides/*genetics Saccharomyces cerevisiae/*genetics *Transcription, Genetic;" |
| Notes: | "MedlineSiliciano, P G Tatchell, K eng Research Support, U.S. Gov't, P.H.S. 1984/07/01 Cell. 1984 Jul; 37(3):969-78. doi: 10.1016/0092-8674(84)90431-8" |
