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J Bacteriol

Title:		A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides
Author(s):		Puskas A; Greenberg EP; Kaplan S; Schaefer AL;
Address:		"Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston 77224, USA"
Journal Title:		J Bacteriol
Year:		1997
Volume:		179
Issue:		23
Page Number:		7530 - 7537
DOI:		10.1128/jb.179.23.7530-7537.1997
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria there are two genes required for quorum sensing, the luxR and luxI homologs, and there is an acylhomoserine lactone signal molecule synthesized by the product of the luxI homolog. In R. sphaeroides, synthesis of a novel homoserine lactone signal, 7,8-cis-N-(tetradecenoyl)homoserine lactone, is directed by a luxI homolog termed cerI. Two open reading frames immediately upstream of cerI are proposed to be components of the quorum-sensing system. The first of these is a luxR homolog termed cerR, and the second is a small open reading frame of 159 bp. Inactivation of cerI in R. sphaeroides results in mucoid colony formation on agar and formation of large aggregates of cells in liquid cultures. Clumping of CerI mutants in liquid culture is reversible upon addition of the acylhomoserine lactone signal and represents a phenotype unlike those controlled by quorum sensing in other bacteria"
Keywords:		"4-Butyrolactone/*analogs & derivatives/biosynthesis/chemistry/pharmacology Amino Acid Sequence Bacterial Proteins/*genetics Base Sequence Cell Communication/*genetics Cloning, Molecular Genes, Bacterial Molecular Sequence Data Pheromones/biosynthesis/chem;"
Notes:		"MedlinePuskas, A Greenberg, E P Kaplan, S Schaefer, A L eng GM15590/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1997/12/11 J Bacteriol. 1997 Dec; 179(23):7530-7. doi: 10.1128/jb.179.23.7530-7537.1997"