| Title: | Post-translational modifications in mitotic yeast cells |
| Author(s): | Nevalainen LT; Louhelainen J; Makarow M; |
| Address: | "Institute of Biotechnology, University of Helsinki, Finland" |
| DOI: | 10.1111/j.1432-1033.1989.tb15003.x |
| ISSN/ISBN: | 0014-2956 (Print) 0014-2956 (Linking) |
| Abstract: | "We have recently shown that secretion of invertase is not inhibited in the yeast Saccharomyces cerevisiae during mitosis, but continues, as during interphase. This is in contrast with the mammalian cell, where membrane traffic stops at the onset of prometaphase. Here we extend our findings by showing that the bulk of the cell surface glycoproteins and mannans, as well as the yeast pheromone alpha-factor, traverse the secretory pathway during mitosis. We show that the mitotic cells are able to carry out several types of post-translational modification of secretory proteins. (a) The secretory protein invertase was oligomerized and extensively glycosylated, (b) the N-glycan cores of bulk-cell surface mannans were extended with outer chains, (c) some N-glycans were phosphorylated, (d) the protein-bound O-glycans were extended up to tetramannosides, (e) prepro-ka-factor was proteolytically processed to alpha-factor molecules. We conclude that the secretory pathway in yeast remains fully functional throughout the cell cycle" |
| Keywords: | "*Cell Cycle Fungal Proteins/biosynthesis/*genetics/isolation & purification Kinetics Mannose/metabolism Membrane Glycoproteins/biosynthesis/*genetics/isolation & purification Mitosis Molecular Weight Polysaccharides/biosynthesis *Protein Processing, Post-;" |
| Notes: | "MedlineNevalainen, L T Louhelainen, J Makarow, M eng Research Support, Non-U.S. Gov't England 1989/09/01 Eur J Biochem. 1989 Sep 1; 184(1):165-72. doi: 10.1111/j.1432-1033.1989.tb15003.x" |
