| Title: | Volatile molecules from bronchoalveolar lavage fluid can 'rule-in' Pseudomonas aeruginosa and 'rule-out' Staphylococcus aureus infections in cystic fibrosis patients |
| Author(s): | Nasir M; Bean HD; Smolinska A; Rees CA; Zemanick ET; Hill JE; |
| Address: | "Geisel School of Medicine, Dartmouth College, 1 Rope Ferry Road, Hanover, NH, 03755, United States. School of Life Sciences, Arizona State University, 427 East Tyler Mall, Tempe, AZ, 85287, United States. NUTRIM School of Nutrition and Translational Research in Metabolism, Department of Pharmacology and Toxicology, Maastricht University, Maastricht, The Netherlands. School of Medicine, Colorado Anschutz Medical Campus and Children's Hospital Colorado, Department of Pediatrics, 13123 E 16th Avenue, Aurora, CO, 80045, United States. Geisel School of Medicine, Dartmouth College, 1 Rope Ferry Road, Hanover, NH, 03755, United States. jane.e.hill@dartmouth.edu. Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, United States. jane.e.hill@dartmouth.edu" |
| DOI: | 10.1038/s41598-017-18491-8 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Respiratory infections caused by Pseudomonas aeruginosa and Staphylococcus aureus are the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. The authors aimed to identify volatile biomarkers from bronchoalveolar lavage (BAL) samples that can guide breath biomarker development for pathogen identification. BAL samples (n = 154) from CF patients were analyzed using two-dimensional gas chromatography time-of-flight mass spectrometry. Random Forest was used to select suites of volatiles for identifying P. aeruginosa-positive and S. aureus-positive samples using multiple infection scenarios and validated using test sets. Using nine volatile molecules, we differentiated P. aeruginosa-positive (n = 7) from P. aeruginosa-negative (n = 53) samples with an area under the receiver operating characteristic curve (AUROC) of 0.86 (95% CI 0.71-1.00) and with positive and negative predictive values of 0.67 (95% CI 0.38-0.75) and 0.92 (95% CI 0.88-1.00), respectively. We were also able to discriminate S. aureus-positive (n = 15) from S. aureus-negative (n = 45) samples with an AUROC of 0.88 (95% CI 0.79-1.00) using eight volatiles and with positive and negative predictive values of 0.86 (95% CI 0.61-0.96) and 0.70 (95% CI 0.61-0.75), respectively. Prospective validation of identified biomarkers as screening tools in patient breath may lead to clinical application" |
| Keywords: | "Adolescent Adult Area Under Curve Biomarkers/analysis Bronchoalveolar Lavage Fluid/*chemistry Child Child, Preschool Cystic Fibrosis/complications/*pathology Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Pseudomonas aeruginosa/chemistry;" |
| Notes: | "MedlineNasir, Mavra Bean, Heather D Smolinska, Agnieszka Rees, Christiaan A Zemanick, Edith T Hill, Jane E eng K23 HL114883/HL/NHLBI NIH HHS/ T32 LM012204/LM/NLM NIH HHS/ UL1 TR001086/TR/NCATS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2018/01/18 Sci Rep. 2018 Jan 16; 8(1):826. doi: 10.1038/s41598-017-18491-8" |
