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Eur J Biochem

Title:		Sex pheromone plasmid pAD1-encoded aggregation substance of Enterococcus faecalis is positively regulated in trans by traE1
Author(s):		Muscholl A; Galli D; Wanner G; Wirth R;
Address:		"Lehrstuhl fur Mikrobiologie, Universitat Munchen, Germany"
Journal Title:		Eur J Biochem
Year:		1993
Volume:		214
Issue:		1
Page Number:		333 - 338
DOI:		10.1111/j.1432-1033.1993.tb17928.x
ISSN/ISBN:		0014-2956 (Print) 0014-2956 (Linking)
Abstract:		"Sex-pheromone-plasmid-bearing strains of Enterococcus faecalis react with sex pheromone to induce the expression of an adhesin, the so-called aggregation substance, on their cell surface. Here we show that, by complementation studies, for sex-pheromone plasmid pAD1, expression of the structural gene asa1, coding for an aggregation substance, is mediated by a diffusible factor encoded on pAD1. We were able to demonstrate that a small open reading frame, traE1, is sufficient for transcription of the operon containing asa1. A model for expression of asa1 under the influence of the positive regulator is presented, which is supported by our observation that regulation involves an all-or-nothing induction phenomenon, leading to cells either fully expressing asa1 or not at all"
Keywords:		"Bacterial Proteins/biosynthesis/*genetics Blotting, Northern Enterococcus faecalis/*genetics/metabolism Gene Expression Regulation, Bacterial Genes, Bacterial *Genes, Regulator *Plasmids Sex Attractants/biosynthesis/*genetics Transcription, Genetic;"
Notes:		"MedlineMuscholl, A Galli, D Wanner, G Wirth, R eng Research Support, Non-U.S. Gov't England 1993/05/15 Eur J Biochem. 1993 May 15; 214(1):333-8. doi: 10.1111/j.1432-1033.1993.tb17928.x"