| Title: | Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism |
| Author(s): | Morbt N; Mogel I; Kalkhof S; Feltens R; Roder-Stolinski C; Zheng J; Vogt C; Lehmann I; von Bergen M; |
| Address: | "Department of Proteomics, UFZ, Helmholtz-Centre for Environmental Research, Leipzig, Germany" |
| ISSN/ISBN: | 1615-9861 (Electronic) 1615-9853 (Print) 1615-9853 (Linking) |
| Abstract: | "Styrene is a volatile organic compound that is widely used as an intermediate in many industrial settings. There are known adverse health effects at environmentally significant concentrations, but little is known about the molecular effect of exposure to styrene at sub-acute toxic concentrations. We exposed human lung epithelial cells, at a wide range of concentrations (1 mg/m(3)-10 g/m(3)), to styrene and analyzed the effects on the proteome level by 2-DE, where 1380 proteins spots were detected and 266 were identified unambiguously by MS. A set of 16 protein spots were found to be significantly altered due to exposure to styrene at environmentally significant concentrations of 1-10 mg/m(3) (0.2-2.3 ppm). Among these, superoxide dismutase as well as biliverdin reductase A could be correlated with the molecular pathway of oxidative stress, while eukaryotic translation initiation factor 5A-1, ezrin, lamin B2 and voltage-dependent anion channel 2 have been reported to be involved in apoptosis. Treatment with styrene also caused the formation of styrene oxide-protein adducts, specifically for thioredoxin reductase 1. These results underline the relevance of oxidative stress as a primary molecular response mechanism of lung epithelial cells to styrene exposure at indoor-relevant concentrations" |
| Keywords: | "Cell Line Cell Survival/drug effects Electrophoresis, Gel, Two-Dimensional Humans Lung/*chemistry/cytology/*drug effects/metabolism Oxidative Stress/*drug effects Proteome/*analysis/metabolism Reactive Oxygen Species/metabolism Styrene/*pharmacology;" |
| Notes: | "MedlineMorbt, Nora Mogel, Iljana Kalkhof, Stefan Feltens, Ralph Roder-Stolinski, Carmen Zheng, Jiang Vogt, Carsten Lehmann, Irina von Bergen, Martin eng R01 HL080226/HL/NHLBI NIH HHS/ R01 HL080226-05/HL/NHLBI NIH HHS/ Research Support, N.I.H., Extramural Germany 2009/10/29 Proteomics. 2009 Nov; 9(21):4920-33. doi: 10.1002/pmic.200800836" |
