| Title: | Tapping into Plant-Microbiome Interactions through the Lens of Multi-Omics Techniques |
| Author(s): | Mishra AK; Sudalaimuthuasari N; Hazzouri KM; Saeed EE; Shah I; Amiri KMA; |
| Address: | "Khalifa Centre for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates. Department of Chemistry (Biochemistry), College of Science, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates. Department of Biology, College of Science, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates" |
| ISSN/ISBN: | 2073-4409 (Electronic) 2073-4409 (Linking) |
| Abstract: | "This review highlights the pivotal role of root exudates in the rhizosphere, especially the interactions between plants and microbes and between plants and plants. Root exudates determine soil nutrient mobilization, plant nutritional status, and the communication of plant roots with microbes. Root exudates contain diverse specialized signaling metabolites (primary and secondary). The spatial behavior of these metabolites around the root zone strongly influences rhizosphere microorganisms through an intimate compatible interaction, thereby regulating complex biological and ecological mechanisms. In this context, we reviewed the current understanding of the biological phenomenon of allelopathy, which is mediated by phytotoxic compounds (called allelochemicals) released by plants into the soil that affect the growth, survival, development, ecological infestation, and intensification of other plant species and microbes in natural communities or agricultural systems. Advances in next-generation sequencing (NGS), such as metagenomics and metatranscriptomics, have opened the possibility of better understanding the effects of secreted metabolites on the composition and activity of root-associated microbial communities. Nevertheless, understanding the role of secretory metabolites in microbiome manipulation can assist in designing next-generation microbial inoculants for targeted disease mitigation and improved plant growth using the synthetic microbial communities (SynComs) tool. Besides a discussion on different approaches, we highlighted the advantages of conjugation of metabolomic approaches with genetic design (metabolite-based genome-wide association studies) in dissecting metabolome diversity and understanding the genetic components of metabolite accumulation. Recent advances in the field of metabolomics have expedited comprehensive and rapid profiling and discovery of novel bioactive compounds in root exudates. In this context, we discussed the expanding array of metabolomics platforms for metabolome profiling and their integration with multivariate data analysis, which is crucial to explore the biosynthesis pathway, as well as the regulation of associated pathways at the gene, transcript, and protein levels, and finally their role in determining and shaping the rhizomicrobiome" |
| Keywords: | *Plant Roots/metabolism Genome-Wide Association Study *Microbiota Soil/chemistry Plants/metabolism Pheromones/metabolism allelopathy ecology metabolomics metagenomics metatranscriptomics plant microbiome plant-microbe interactions primary metabolites rhiz; |
| Notes: | "MedlineMishra, Ajay Kumar Sudalaimuthuasari, Naganeeswaran Hazzouri, Khaled M Saeed, Esam Eldin Shah, Iltaf Amiri, Khaled M A eng Research Support, Non-U.S. Gov't Review Switzerland 2022/10/28 Cells. 2022 Oct 17; 11(20):3254. doi: 10.3390/cells11203254" |
