| Title: | Pre-analytical and analytical variables that influence urinary volatile organic compound measurements |
| Author(s): | McFarlan EM; Mozdia KE; Daulton E; Arasaradnam R; Covington J; Nwokolo C; |
| Address: | "Department of Gastroenterology, University Hospitals Coventry and Warwickshire, Coventry, United Kingdom. School of Engineering, University of Warwick, Coventry, United Kingdom. Department of Health Sciences, University of Leicester, United Kingdom. Faculty of Health Science, University of Coventry, United Kingdom" |
| DOI: | 10.1371/journal.pone.0236591 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "There has been rapidly accelerating interest in the utilization of volatile organic compounds (VOCs) as non-invasive methods for rapid point-of-care medical diagnostics. There is widespread variation in analytical methods and protocols, with little understanding of the effects of sample storage on VOC profiles. This study aimed to determine the effects on VOC profiles of different storage times, at room temperature, prior to freezing, of sealed urine samples from healthy individuals. Analysis using Field Asymmetric Ion Motility Spectrometry (FAIMS) determined the alterations in VOC and total ion count profiles as a result of increasing room temperature storage times. Results indicated that increasing exposure time to room temperature prior to freezing had a threefold effect. Firstly, increased urinary VOC profile variability, with a plateau phase between 12 and 48 hours, before further degradation. Secondly, an increase in total ion count with time exposed to room temperature. Finally, a deterioration in VOCs with each sample run during the analysis process. This provides new insight into the effect of storage of urine samples for VOC analysis using FAIMS technology. Results of this study provide a recommendation for a 12-hour maximum duration at room temperature prior to storage" |
| Keywords: | Female Healthy Volunteers Humans Male Middle Aged Temperature Time Factors Urinalysis/*methods Volatile Organic Compounds/*urine; |
| Notes: | "MedlineMcFarlanE, Michael MozdiaK, Ella Daulton, Emma Arasaradnam, Ramesh Covington, James Nwokolo, Chuka eng 2020/08/01 PLoS One. 2020 Jul 31; 15(7):e0236591. doi: 10.1371/journal.pone.0236591. eCollection 2020" |
