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Ecol Evol

Title:		Transcriptome analysis and molecular characterization of soluble chemical communication proteins in the parasitoid wasp Anagrus nilaparvatae (Hymenoptera: Mymaridae)
Author(s):		Ma Y; Huang T; Tang B; Wang B; Wang L; Liu J; Zhou Q;
Address:		State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Guangzhou China. School of Agriculture Sun Yat-Sen University Guangzhou China. Institute of Nanfan & Seed Industry Guangdong Academy of Sciences Guangzhou China
Journal Title:		Ecol Evol
Year:		2022
Volume:		20220301
Issue:		3
Page Number:		e8661 -
DOI:		10.1002/ece3.8661
ISSN/ISBN:		2045-7758 (Print) 2045-7758 (Electronic) 2045-7758 (Linking)
Abstract:		"Anagrus nilaparvatae is an important egg parasitoid wasp of pests such as the rice planthopper. Based on the powerful olfactory system of sensing chemical information in nature, A. nilaparvatae shows complicated life activities and behaviors, such as feeding, mating, and hosting. We constructed a full-length transcriptome library and used this to identify the characteristics of soluble chemical communication proteins. Through full-length transcriptome sequencing, splicing, assembly, and data correction by Illumina, we obtained 163.59 Mb of transcriptome data and 501,179 items with annotation information. We then performed Gene Ontology (GO) functional classification of the transcriptome's unigenes. We analyzed the sequence characteristics of soluble chemical communication protein genes and identified eight genes: AnilOBP2, AnilOBP9, AnilOBP23, AnilOBP56, AnilOBP83, AnilCSP5, AnilCSP6, and AnilNPC2. After sequence alignment and conserved domain prediction, the eight proteins encoded by the eight genes above were found to be consistent with the typical characteristics of odorant-binding proteins (OBPs), chemosensory proteins (CSPs), and Niemann-pick type C2 proteins (NPC2s) in other insects. Phylogenetic tree analysis showed that the eight genes share low homology with other species of Hymenoptera. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to analyze the expression responses of the eight genes in different sexes and upon stimulation by volatile organic compounds. The relative expression levels of AnilOBP9, AnilOBP26, AnilOBP83, AnilCSP5, and AnilNPC2 in males were significantly higher than those in females, while the relative expression level of AnilCSP6 was higher in females. The expression levels of AnilOBP9 and AnilCSP6 were significantly altered by the stimulation of beta-caryophyllene, suggesting that these two genes may be related to host detection. This study provides the first data for A. nilaparvatae's transcriptome and the molecular characteristics of soluble chemical communication proteins, as well as an opportunity for understanding how A. nilaparvatae behaviors are mediated via soluble chemical communication proteins"
Keywords:		Anagrus nilaparvatae Niemann-pick type C2 proteins chemosensory proteins odorant-binding proteins transcriptome;
Notes:		"PubMed-not-MEDLINEMa, Ying Huang, Tingfa Tang, Bingjie Wang, Bingyang Wang, Liyang Liu, Jianbai Zhou, Qiang eng England 2022/03/10 Ecol Evol. 2022 Mar 1; 12(3):e8661. doi: 10.1002/ece3.8661. eCollection 2022 Feb"