| Title: | Transcriptomic analyses of chemosensory genes in Trichogramma japonicum (Hymenoptera: Trichogrammatidae) |
| Author(s): | Li SS; Yan ZC; Zhao JJ; Li YX; |
| Address: | "Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China. Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: yxli@njau.edu.cn" |
| Journal Title: | Comp Biochem Physiol Part D Genomics Proteomics |
| DOI: | 10.1016/j.cbd.2020.100755 |
| ISSN/ISBN: | 1878-0407 (Electronic) 1744-117X (Linking) |
| Abstract: | "Insects perceive dangerous or attractive chemicals in the environment (such as the presence of predators, food or mates) through their olfaction and gustation. This leads to host searching, mate finding and other behaviors that are critical for insect survival. These vital activities are mediated by many chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs). Trichogramma japonicum Ashmead (Hymenoptera: Trichogrammatidae) is a commonly used egg parasitoid for controlling lepidopteran pests in rice fields. To reveal the roles of its olfactory and gustatory systems in biological control, we systematically analyzed major chemosensory receptor genes from T. japonicum. Through transcriptomic analyses of male and female heads, we found that the differentially expressed genes (DEGs) were mainly related to chemosensory functions, and there were more chemosensory-related genes expressed in female heads than male heads, which may be related to the need for females to search for their hosts or sense male pheromone. Furthermore, we identified 66 chemosensory receptor genes, including 51 ORs, seven IRs and eight GRs, that were most closely relate to those of other hymenopteran species according to the results of both Blast best-hit and phylogenetic analyses. The tissue expression profile showed that 65 of the 66 chemosensory receptors were highly expressed in the heads, suggesting their putative roles in olfaction and gustation. In addition, the sex-specific expression patterns suggested their potential functions in host-seeking or mate sensing behaviors. This study may provide base for further understanding the olfactory and gustatory systems of T. japonicum and increasing its efficiency in pest control in the future" |
| Keywords: | "Animals Female Gene Expression Profiling Genes, Insect Hymenoptera/*genetics Insect Proteins/genetics Male Phylogeny Receptors, Cell Surface/genetics Receptors, Odorant/genetics *Transcriptome Gustatory receptor Ionotropic receptor Odorant receptor RT-qPC;" |
| Notes: | "MedlineLi, Si-Sheng Yan, Zhi-Chao Zhao, Juan-Juan Li, Yuan-Xi eng Research Support, Non-U.S. Gov't Netherlands 2020/11/10 Comp Biochem Physiol Part D Genomics Proteomics. 2021 Mar; 37:100755. doi: 10.1016/j.cbd.2020.100755. Epub 2020 Oct 29" |
