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Anal Bioanal Chem

Title:		"Simultaneous determination of urinary 31 metabolites of VOCs, 8-hydroxy-2'-deoxyguanosine, and trans-3'-hydroxycotinine by UPLC-MS/MS: (13)C- and (15)N-labeled isotoped internal standards are more effective on reduction of matrix effect"
Author(s):		Kuang H; Li Y; Jiang W; Wu P; Tan J; Zhang H; Pang Q; Ma S; An T; Fan R;
Address:		"Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, School of Life Sciences, South China Normal University, Guangzhou, 510631, Guangdong, China. Guangzhou Women and Children's Medical Center, Guangzhou, 510120, Guangdong, China. Guangzhou Quality Supervision and Testing Institute, Guangzhou, 511447, Guangdong, China. Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, Guangdong, China. Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, School of Life Sciences, South China Normal University, Guangzhou, 510631, Guangdong, China. 20001047@m.scnu.edu.cn"
Journal Title:		Anal Bioanal Chem
Year:		2019
Volume:		20191120
Issue:		29
Page Number:		7841 - 7855
DOI:		10.1007/s00216-019-02202-5
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Human beings are inevitably exposed to volatile organic compounds (VOCs) of anthropogenic emissions as they are ubiquitous atmospheric pollutants. Smoking is an important exposure route of VOCs for the general population. Health effects induced by VOC exposure raise more concerns as they are identified with carcinogenicity, genotoxicity, neurotoxicity, and reproductive toxicity. trans-3'-Hydroxycotinine (OH-Cot) is a urinary biomarker of smoking, and 8-hydroxy-2'-deoxyguanosine (8-OHDG) is a urinary biomarker of DNA oxidative damage. To develop a method for quantifying VOC exposure levels of the general population and assessing the health risks induced by VOCs from second-hand smoking, an effective, rapid, and high-throughput method for the simultaneous determination of 31 metabolites of VOCs, 8-OHDG, and OH-Cot using solid-phase extraction coupled with UPLC-MS/MS was developed and validated. Method precision and accuracy, extraction recoveries, matrix effects, and storage stabilities of most analytes met the criterion (80-120%). Extraction recoveries increased from 85.1 to 100% after adjustment by isotoped internal standards (ISs). Furthermore, (13)C- and (15)N-labeled ISs were more effective to reduce the influence of matrix effects on recoveries and precisions than the deuterated analogs (73.0-116% vs. 53.6-140%). This developed method was successfully applied to determine urine samples collected from children. Results showed that N-acetyl-S-(3,4-dihydrobutyl)-L-cysteine, 2,2'-thiodiacetic acid (TGA), and N-acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (HPMMA) were well correlated with 8-OHDG with coefficients higher than 0.82, indicating those VOCs might easily lead to DNA damage. In conclusion, our co-monitoring of metabolites of VOCs with 8-OHDG and OH-Cot in one method provides a robust analytical method, which not only suggests the potential adverse health effects induced by VOCs but also discriminates and evaluates the contribution of passive smoking in human VOC exposure. Graphical abstract"
Keywords:		"8-Hydroxy-2'-Deoxyguanosine/standards/*urine Case-Control Studies Chromatography, Liquid/*methods Cotinine/*analogs & derivatives/standards/urine Humans Nitrogen Isotopes Reference Standards Smoking/urine Tandem Mass Spectrometry/*methods Volatile Organic;"
Notes:		"MedlineKuang, Hongxuan Li, Yonghong Jiang, Wenhui Wu, Peiqiong Tan, Jianhua Zhang, Haibin Pang, Qihua Ma, Shengtao An, Taicheng Fan, Ruifang eng 41731279/National Natural Science Foundation of China/ Germany 2019/11/22 Anal Bioanal Chem. 2019 Nov; 411(29):7841-7855. doi: 10.1007/s00216-019-02202-5. Epub 2019 Nov 20"