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J Biol Chem

Title:		Plant phenylacetaldehyde synthase is a bifunctional homotetrameric enzyme that catalyzes phenylalanine decarboxylation and oxidation
Author(s):		Kaminaga Y; Schnepp J; Peel G; Kish CM; Ben-Nissan G; Weiss D; Orlova I; Lavie O; Rhodes D; Wood K; Porterfield DM; Cooper AJ; Schloss JV; Pichersky E; Vainstein A; Dudareva N;
Address:		"Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010, USA"
Journal Title:		J Biol Chem
Year:		2006
Volume:		20060609
Issue:		33
Page Number:		23357 - 23366
DOI:		10.1074/jbc.M602708200
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"We have isolated and characterized Petunia hybrida cv. Mitchell phenylacetaldehyde synthase (PAAS), which catalyzes the formation of phenylacetaldehyde, a constituent of floral scent. PAAS is a cytosolic homotetrameric enzyme that belongs to group II pyridoxal 5'-phosphate-dependent amino-acid decarboxylases and shares extensive amino acid identity (approximately 65%) with plant L-tyrosine/3,4-dihydroxy-L-phenylalanine and L-tryptophan decarboxylases. It displays a strict specificity for phenylalanine with an apparent Km of 1.2 mM. PAAS is a bifunctional enzyme that catalyzes the unprecedented efficient coupling of phenylalanine decarboxylation to oxidation, generating phenylacetaldehyde, CO2, ammonia, and hydrogen peroxide in stoichiometric amounts"
Keywords:		Acetaldehyde/*analogs & derivatives/chemistry/metabolism Amino Acid Sequence Catalysis Decarboxylation Molecular Sequence Data Multienzyme Complexes/*chemistry/genetics/isolation & purification Oxidation-Reduction Petunia/*enzymology/genetics Phenylalanin;
Notes:		"MedlineKaminaga, Yasuhisa Schnepp, Jennifer Peel, Greg Kish, Christine M Ben-Nissan, Gili Weiss, David Orlova, Irina Lavie, Orly Rhodes, David Wood, Karl Porterfield, D Marshall Cooper, Arthur J L Schloss, John V Pichersky, Eran Vainstein, Alexander Dudareva, Natalia eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2006/06/13 J Biol Chem. 2006 Aug 18; 281(33):23357-66. doi: 10.1074/jbc.M602708200. Epub 2006 Jun 9"