| Title: | The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles |
| Author(s): | Johnston GC; Prendergast JA; Singer RA; |
| Address: | "Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada" |
| ISSN/ISBN: | 0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking) |
| Abstract: | "After the initiation of bud formation, cells of the yeast Saccharomyces cerevisiae direct new growth to the developing bud. We show here that this vectorial growth is facilitated by activity of the MYO2 gene. The wild-type MYO2 gene encodes an essential form of myosin composed of an NH2-terminal domain typical of the globular, actin-binding domain of other myosins. This NH2-terminal domain is linked by what appears to be a short alpha-helical domain to a novel COOH-terminal region. At the restrictive temperature the myo2-66 mutation does not impair DNA, RNA, or protein biosynthetic activity, but produces unbudded, enlarged cells. This phenotype suggests a defect in localization of cell growth. Measurements of cell size demonstrated that the continued development of initiated buds, as well as bud initiation itself, is inhibited. Bulk secretion continues in mutant cells, although secretory vesicles accumulate. The MYO2 myosin thus may function as the molecular motor to transport secretory vesicles along actin cables to the site of bud development" |
| Keywords: | "Actins/analysis Amino Acid Sequence Base Sequence Chitin/metabolism Cytoplasmic Granules/metabolism Cytoskeleton/ultrastructure Genes, Fungal Glycoside Hydrolases/metabolism Mating Factor Microscopy, Electron Microtubules/ultrastructure Molecular Sequence;" |
| Notes: | "MedlineJohnston, G C Prendergast, J A Singer, R A eng Research Support, Non-U.S. Gov't 1991/05/01 J Cell Biol. 1991 May; 113(3):539-51. doi: 10.1083/jcb.113.3.539" |
