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Appl Microbiol Biotechnol


Title:"Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry"
Author(s):Cardenas N; Arroyo R; Calzada J; Peiroten A; Medina M; Rodriguez JM; Fernandez L;
Address:"Probisearch, 28760 Tres Cantos, Madrid, Spain. Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain. Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Carretera de La Coruna, km. 7.5, 28040, Madrid, Spain. Probisearch, 28760 Tres Cantos, Madrid, Spain. leonides@ucm.es. Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain. leonides@ucm.es"
Journal Title:Appl Microbiol Biotechnol
Year:2016
Volume:20160523
Issue:17
Page Number:7665 - 7677
DOI: 10.1007/s00253-016-7616-3
ISSN/ISBN:1432-0614 (Electronic) 0175-7598 (Linking)
Abstract:"In this work, a variety of biochemical properties of Enterococcus faecium CECT 8849, which had been isolated from breast milk, were analyzed. Its acidifying capacity and proteolytic activity were low but, in contrast, remarkable peptidase and esterase activities were observed. Ethanol and 3-hydroxy-2-butanone were the most abundant volatile compounds found in experimental model cheese manufactured with E. faecium CECT 8849. This strain inhibited the growth of several Listeria monocytogenes and Listeria innocua strains in vitro. Enterocin A and B structural genes were detected in E. faecium CECT 8849. Model fermented milk and cheeses were manufactured from milk inoculated or not with L. innocua CECT 8848 (2.5-3 log10 colony forming units mL(-1)) using E. faecium CECT 8849 or Lactococcus lactis ESI 153 as starter cultures. Although E. faecium CECT 8849 controlled Listeria growth in both dairy models, it led to lower reduction in Listeria counts when compared with L. lactis ESI 153"
Keywords:Acetoin/metabolism Bacteriocins/genetics Cheese/*microbiology Cultured Milk Products/*microbiology Dairying Enterococcus faecium/*enzymology/genetics/isolation & purification/*metabolism Esterases/*metabolism Ethanol/analysis Food Microbiology Humans Lact;
Notes:"MedlineCardenas, Nivia Arroyo, Rebeca Calzada, Javier Peiroten, Angela Medina, Margarita Rodriguez, Juan Miguel Fernandez, Leonides eng Germany 2016/05/25 Appl Microbiol Biotechnol. 2016 Sep; 100(17):7665-77. doi: 10.1007/s00253-016-7616-3. Epub 2016 May 23"

 
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