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Aquat Toxicol

Title:		Proteome response of fish under multiple stress exposure: Effects of pesticide mixtures and temperature increase
Author(s):		Gandar A; Laffaille P; Marty-Gasset N; Viala D; Molette C; Jean S;
Address:		"EcoLab, Universite de Toulouse, CNRS, INPT, UPS, Toulouse, France. GenPhySE, Universite de Toulouse, INRA, INPT, ENVT, 31326 Castanet-Tolosan, France. Plate-Forme 'Exploration du Metabolisme', Centre de Clermont-Ferrand, Theix, 63122, Saint Genes Champanelle, France; UMR 1213 Herbivores, INRA, VetAgro Sup, NRA Theix, 63122, Saint Genes Champanelle, France. EcoLab, Universite de Toulouse, CNRS, INPT, UPS, Toulouse, France. Electronic address: severine.jean@ensat.fr"
Journal Title:		Aquat Toxicol
Year:		2017
Volume:		20170115
Issue:
Page Number:		61 - 77
DOI:		10.1016/j.aquatox.2017.01.004
ISSN/ISBN:		1879-1514 (Electronic) 0166-445X (Linking)
Abstract:		"Aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. Evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. To the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. For that, goldfish (Carassius auratus) were acclimated to two different temperatures (22 and 32 degrees C) for 15 days. They were then exposed for 96h to a cocktail of herbicides and fungicides (S-metolachlor, isoproturon, linuron, atrazine-desethyl, aclonifen, pendimethalin and tebuconazole) at two environmentally relevant concentrations (total concentrations of 8.4mugL(-1) and 42mugL(-1)) at these two temperatures (22 and 32 degrees C). The molecular response in liver was assessed by 2D-proteomics. Identified proteins were integrated using pathway enrichment analysis software to determine the biological functions involved in the individual or combined stress responses and to predict the potential deleterious outcomes. The pesticide mixtures elicited pathways involved in cellular stress response, carbohydrate, protein and lipid metabolisms, methionine cycle, cellular functions, cell structure and death control, with concentration- and temperature-dependent profiles of response. We found that combined temperature increase and pesticide exposure affected the cellular stress response: the effects of oxidative stress were more marked and there was a deregulation of the cell cycle via apoptosis inhibition. Moreover a decrease in the formation of glucose by liver and in ketogenic activity was observed in this multi-stress condition. The decrease in both pathways could reflect a shift from a metabolic compensation strategy to a conservation state. Taken together, our results showed (1) that environmental cocktails of herbicides and fungicides induced important changes in pathways involved in metabolism, cell structure and cell cycle, with possible deleterious outcomes at higher biological scales and (2) that increasing temperature could affect the response of fish to pesticide exposure"
Keywords:		"Animals Fungicides, Industrial/toxicity Goldfish/metabolism/*physiology Oxidative Stress/*drug effects Pesticides/metabolism/*toxicity Proteome/*drug effects *Temperature Water Pollutants, Chemical/toxicity Biological pathways Carassius auratus Herbicides;"
Notes:		"MedlineGandar, Allison Laffaille, Pascal Marty-Gasset, Nathalie Viala, Didier Molette, Caroline Jean, Severine eng Netherlands 2017/01/23 Aquat Toxicol. 2017 Mar; 184:61-77. doi: 10.1016/j.aquatox.2017.01.004. Epub 2017 Jan 15"