| Title: | "Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating, DNA-binding TraA protein" |
| Address: | "Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "The Enterococcus faecalis conjugative plasmid pAD1 (60 kb) encodes a mating response to the recipient-produced peptide sex pheromone cAD1. The response involves two key plasmid-encoded regulatory proteins: TraE1, which positively regulates all or most structural genes relating to conjugation, and TraA, which binds DNA and negatively regulates expression of traE1. In vitro studies that included development of a DNA-associated protein-tag affinity chromatography technique showed that TraA (37.9 kDa) binds directly to cAD1 near its carboxyl-terminal end and, as a consequence, loses its affinity for DNA. Analyses of genetically modified TraA proteins indicated that truncations within the carboxyl-terminal 9 residues significantly affected the specificity of peptide-directed association/dissociation of DNA. The data support earlier observations that transposon insertions near the 3' end of traA eliminated the ability of cells to respond to cAD1" |
| Keywords: | "Bacterial Outer Membrane Proteins/*metabolism Carboxy-Lyases/*metabolism DNA, Bacterial/genetics/*metabolism DNA-Binding Proteins/metabolism Enterococcus faecalis/*metabolism Fimbriae Proteins Gene Expression Regulation, Bacterial;" |
| Notes: | "MedlineFujimoto, S Clewell, D B eng M01 RR000042/RR/NCRR NIH HHS/ GM33956/GM/NIGMS NIH HHS/ MO1-RR00042/RR/NCRR NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1998/05/30 Proc Natl Acad Sci U S A. 1998 May 26; 95(11):6430-5. doi: 10.1073/pnas.95.11.6430" |
