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Proc Natl Acad Sci U S A

Title:		Sex pheromone desaturase functioning in a primitive Ostrinia moth is cryptically conserved in congeners' genomes
Author(s):		Fujii T; Ito K; Tatematsu M; Shimada T; Katsuma S; Ishikawa Y;
Address:		"Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2011
Volume:		20110328
Issue:		17
Page Number:		7102 - 7106
DOI:		10.1073/pnas.1019519108
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"(E)-11- and (Z)-11-tetradecenyl acetate are the most common female sex pheromone components in Ostrinia moths. The Delta11-desaturase expressed in the pheromone gland (PG) of female moths is a key enzyme that introduces a double bond into pheromone molecules. A single Delta11-desaturase of Ostrinia nubilalis, OnubZ/E11, has been shown to produce an approximately 7:3 mixture of (E)-11- and (Z)-11-tetradecenoate from the substrate tetradecanoate. In contrast, the sex pheromone of Ostrinia latipennis, a primitive species of Ostrinia, is (E)-11-tetradecenol. This pheromone is unique in that it is not acetylated, and includes no Z isomer. In the present study, through the cloning and functional analysis of a PG-specific Delta11-desaturase in O. latipennis, we showed that the absence of the Z isomer in the pheromone is attributable to the strict product specificity of the Delta11-desaturase in this species, LATPG1. Phylogenetic analysis revealed that LATPG1 was not closely related to OnubZ/E11. Rather, it was closely related to retroposon-linked cryptic Delta11-desaturases (ezi-Delta11) found in the genomes of O. nubilalis and Ostrinia furnacalis. Taken together, the results showed that an unusual Delta11-desaturase is functionally expressed in O. latipennis, although the genes encoding this enzyme appear to be cryptic in congeners"
Keywords:		"Acetylation Amino Acid Sequence Animals Cloning, Molecular Fatty Acid Desaturases/genetics/*metabolism Female Genome, Insect/*physiology Insect Proteins/genetics/*metabolism Male Molecular Sequence Data Moths/*enzymology/genetics *Phylogeny Sex Attractant;"
Notes:		"MedlineFujii, Takeshi Ito, Katsuhiko Tatematsu, Mitsuko Shimada, Toru Katsuma, Susumu Ishikawa, Yukio eng Research Support, Non-U.S. Gov't 2011/03/30 Proc Natl Acad Sci U S A. 2011 Apr 26; 108(17):7102-6. doi: 10.1073/pnas.1019519108. Epub 2011 Mar 28"