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Biofouling

Title:		"Purification and partial amino acid sequence analysis of the larval settlement-inducing pheromone from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite)"
Author(s):		Endo N; Nogata Y; Yoshimura E; Matsumura K;
Address:		"Central Research Institute of Electric Power Industry, Abiko-shi, Chiba-ken, Japan. n-endo@criepi.denken.or.jp"
Journal Title:		Biofouling
Year:		2009
Volume:		25
Issue:		5
Page Number:		429 - 434
DOI:		10.1080/08927010902875113
ISSN/ISBN:		1029-2454 (Electronic) 0892-7014 (Linking)
Abstract:		"A previously undescribed larval settlement-inducing protein was purified from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite). Results of SDS-PAGE indicated that the relative molecular mass of the protein in reduced and denatured form is 31,600 +/- 500 kDa, and that it is distinct from the Settlement Inducing Protein Complex (SIPC) which has previously been determined as a larval settlement-inducing pheromone. The N-terminal 33-residue sequence of the intact protein showed no similarity with previously reported proteins in the EMBL/Genbank/DDBJ databases. The purified protein at a concentration of 10 microg ml(-1) induced approximately four times more larval settlement than the control (filtered natural seawater). In addition, results of the assay using both 24-well polystyrene plates and agarose gels indicated that this protein is probably released into seawater and attracts cypris larvae. These results suggest that the purified protein is a waterborne type pheromone which induces settlement of larvae of B. amphitrite"
Keywords:		"Amino Acid Sequence Animals *Behavior, Animal Electrophoresis, Polyacrylamide Gel Larva/growth & development Molecular Sequence Data Molecular Weight Pheromones/chemistry/*isolation & purification Thoracica/*growth & development/metabolism;"
Notes:		"MedlineEndo, Noriyuki Nogata, Yasuyuki Yoshimura, Erina Matsumura, Kiyotaka eng England 2009/03/27 Biofouling. 2009; 25(5):429-34. doi: 10.1080/08927010902875113"