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Gene

Title:		Secretion of glycosylated human erythropoietin from yeast directed by the alpha-factor leader region
Author(s):		Elliott S; Giffin J; Suggs S; Lau EP; Banks AR;
Address:		"Amgen, Thousand Oaks, CA 91320"
Journal Title:		Gene
Year:		1989
Volume:		79
Issue:		1
Page Number:		167 - 180
DOI:		10.1016/0378-1119(89)90102-9
ISSN/ISBN:		0378-1119 (Print) 0378-1119 (Linking)
Abstract:		"The pre-pro alpha-factor leader region of the yeast MF alpha 1 gene was used to direct the secretion of the human glycoprotein, erythropoietin (EPO), into the culture medium. An examination of the role of expression level on secretion of biologically active EPO indicated that there are several rate-limiting steps. These include processing of the alpha-factor-EPO precursor protein by the KEX2-encoded endoproteinase and transport of the protein through the secretory pathway. The rate-limiting steps for transport were early in the secretory pathway, probably from the endoplasmic reticulum to the Golgi apparatus"
Keywords:		"Animals Biological Transport DNA, Fungal/genetics Endopeptidases/metabolism Erythropoietin/genetics/*metabolism Gene Amplification Genes, Synthetic Glycosylation Humans Immunoblotting Mating Factor Peptides/*genetics/metabolism Plasmids Promoter Regions, ;"
Notes:		"MedlineElliott, S Giffin, J Suggs, S Lau, E P Banks, A R eng Netherlands 1989/06/30 Gene. 1989 Jun 30; 79(1):167-80. doi: 10.1016/0378-1119(89)90102-9"