| Title: | Optimization of microwave-assisted extraction followed by solid phase micro extraction and gas chromatography-mass spectrometry detection for the assay of some semi volatile organic pollutants in sebum |
| Author(s): | Diaz-Vazquez LM; Garcia O; Velazquez Z; Marrero I; Rosario O; |
| Address: | "Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, P.O. Box 23346, San Juan 00931-3346, Puerto Rico. lizvazquez8@hotmail.com" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2005.05.047 |
| ISSN/ISBN: | 1570-0232 (Print) 1570-0232 (Linking) |
| Abstract: | "Methodology using MAE/SPME/GC-MS is being pursued for the analysis of organic pollutants in sebum. The microwave-assisted extraction (MAE) of standards of semi volatile organic pollutants from sebum was optimized. All compounds were extracted from sebum with recoveries analyzed by GC/MS ranging from 94% to 100% under the optimum MAE conditions: 10mL acetone-hexane (2:1), 60 degrees C, and 10 min microwave heating. To improve the detection limits a SPME procedure was optimized. Linearity ranged from 0.70 ppb to 25 ppb. R.S.D. were in the range of 1-23% for the SPME step. Preliminary real samples were analyzed and a range of compounds was detected. The optimized MAE/SPME/GC-MS methodology promises to be useful for different applications" |
| Keywords: | Environmental Pollutants/*analysis Gas Chromatography-Mass Spectrometry/*methods Indicators and Reagents *Microwaves Sebum/*chemistry Sensitivity and Specificity Volatilization; |
| Notes: | "MedlineDiaz-Vazquez, Liz M Garcia, Oscar Velazquez, Zorangelys Marrero, Ivelisse Rosario, Osvaldo eng Research Support, Non-U.S. Gov't Netherlands 2005/08/23 J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 15; 825(1):11-20. doi: 10.1016/j.jchromb.2005.05.047" |
