| Title: | Phenotypic plasticity in three Daphnia genotypes in response to predator kairomone: evidence for an involvement of chitin deacetylases |
| Author(s): | Christjani M; Fink P; von Elert E; |
| Address: | "Cologne Biocenter, University of Cologne, Zulpicher Strasse 47b, 50674 Cologne, Germany m_christjani@gmx.de. Cologne Biocenter, University of Cologne, Zulpicher Strasse 47b, 50674 Cologne, Germany" |
| ISSN/ISBN: | 1477-9145 (Electronic) 0022-0949 (Linking) |
| Abstract: | "The genetic background of inducible morphological defences in Daphnia is still largely unknown. Dissolved infochemicals from the aquatic larvae of the phantom midge Chaoborus induce so-called 'neck-teeth' in the first three post-embryonic stages of Daphnia pulex This defence has become a textbook example of inducible defences. In a target gene approach, by using three Daphnia genotypes which show a gradient of neck-teeth induction in response to equal amounts of kairomone, we report a high correlation of neck-teeth induction in Daphnia pulex and relative gene expression of two chitin deacetylases. Further, previous studies suggested genes from both the juvenoid and the insulin hormone signalling pathways as well as several morphogenetic genes downstream to be responsible for neck-teeth induction in D. pulex However, these data were not supported by our study. None of the three D. pulex clones showed an upregulation of these previously proposed candidate genes as a response to predator kairomone, which is interpreted as the result of refined methods used for both RNA sampling and kairomone enrichment yielding unambiguous results compared with earlier studies. The assessment of a clonal gradient of Daphnia in the presence and absence of infochemicals provides a promising approach to identify further genes involved in the induction of morphological defences by correlating gene expression and morphology" |
| Keywords: | "Amidohydrolases/genetics/*metabolism Animals Clone Cells Daphnia/enzymology/*genetics/immunology/*physiology Gene Expression Regulation, Enzymologic/drug effects Genetic Association Studies Genotype Larva/drug effects/physiology Phenotype Pheromones/*phar;" |
| Notes: | "MedlineChristjani, Mark Fink, Patrick von Elert, Eric eng England 2016/03/20 J Exp Biol. 2016 Jun 1; 219(Pt 11):1697-704. doi: 10.1242/jeb.133504. Epub 2016 Mar 18" |
