| Title: | "Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae" |
| Author(s): | Chow TY; Ash JJ; Dignard D; Thomas DY; |
| Address: | "Genetic Engineering Section, NRCC-BRI, Montreal, Quebec, Canada" |
| ISSN/ISBN: | 0021-9533 (Print) 0021-9533 (Linking) |
| Abstract: | "Utilizing a screening method designed for the identification of genes involved with enhanced protein secretion in Saccharomyces cerevisiae we identified a gene, which we named PSE-1 (Protein Secretion Enhancer). Overexpression of PSE-1 in a multi-copy plasmid, as shown by Northern hybridization, gave a fourfold enhancement in total protein secretion. The repertoire of proteins that are found to be secreted in greater quantities include three known biologically active proteins: k1 killer toxin, alpha-factor, and acid phosphatase. The PSE-1 gene is located on chromosome XII of the yeast genome and codes for a hydrophobic protein containing 1089 amino acids. Haploid yeast cells that contained a LEU2 insertion mutation in PSE-1 grow very poorly, a phenotype similar to other conditional SEC mutants at restrictive temperature" |
| Keywords: | "Acid Phosphatase/metabolism Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular DNA, Fungal Electrophoresis, Polyacrylamide Gel Fungal Proteins/*genetics/metabolism Genes, Fungal Mating Factor *Membrane Transport Proteins Molecular Seq;" |
| Notes: | "MedlineChow, T Y Ash, J J Dignard, D Thomas, D Y eng Research Support, Non-U.S. Gov't England 1992/03/01 J Cell Sci. 1992 Mar; 101 ( Pt 3):709-19. doi: 10.1242/jcs.101.3.709" |
