| Title: | Use of Yellow Fluorescent Protein Fluorescence to Track OPR3 Expression in Arabidopsis Thaliana Responses to Insect Herbivory |
| Author(s): | Body MJA; Dave DF; Coffman CM; Paret TY; Koo AJ; Cocroft RB; Appel HM; |
| Address: | "Division of Plant Sciences, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, United States. Division of Biochemistry, University of Missouri, Columbia, MO, United States. Division of Biological Sciences, University of Missouri, Columbia, MO, United States" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Feeding by chewing insects induces chemical defenses in plants that are regulated by the jasmonic acid (JA) pathway. Jasmonates are usually quantified by liquid chromatography-mass spectrometry (LC-MS) analysis of precursors and products in the biosynthetic pathway or inferred from the extraction and expression of genes known to respond to elevated levels of JA. Both approaches are costly and time consuming. To address these limitations, we developed a rapid reporter for the synthesis of JA based on the OPR3promoter:YFP-PTS1. Yellow fluorescent protein (YFP) fluorescence was increased by mechanical wounding and methyl jasmonate (MeJA) treatment and by caterpillar feeding. To develop an optimal sampling time for a quantitative bioassay, OPR3promoter:YFP-PTS1 plants were sampled at 1, 2, 3, and 24 h after treatment with 115 microM MeJA. The first increase in YFP fluorescence was detected at 2 h and remained elevated 3 and 24 h later; as a result, 3 h was chosen as the sampling time for a quantitative bioassay of jasmonate response to insect attack. Feeding by Pieris rapae caterpillars induced a 1.8-fold increase in YFP fluorescence, consistent with the known induction of JA production by this insect. We also assessed the utility of this reporter in studies of plant responses to caterpillar feeding vibrations, which are known to potentiate the JA-dependent production of chemical defenses. Pretreatment with feeding vibrations increased expression of the OPR3promoter:YFP-PTS1 in response to 14 microM MeJA. Feeding vibrations did not potentiate responses at higher MeJA concentrations, suggesting that potentiating effects of prior treatments can only be detected when plants are below a response threshold to the elicitor. The expression of OPR3 does not indicate levels of specific downstream jasmonates and quantification of specific jasmonates still requires detailed analysis by LC-MS. However, OPR3 expression does provide a rapid and inexpensive way to screen large numbers of plants for the involvement of jasmonate signaling in their response to a wide variety of treatments, and to study the induction and expression of AtOPR3" |
| Keywords: | Arabidopsis thaliana Opr3 caterpillar feeding vibrations herbivory jasmonic acid; |
| Notes: | "PubMed-not-MEDLINEBody, Melanie J A Dave, Dhruveesh F Coffman, Clayton M Paret, Taylor Y Koo, Abraham J Cocroft, Reginald B Appel, Heidi M eng Switzerland 2019/12/19 Front Plant Sci. 2019 Nov 29; 10:1586. doi: 10.3389/fpls.2019.01586. eCollection 2019" |
