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Anal Chim Acta

Title:		Use of exogenous volatile organic compounds to detect Salmonella in milk
Author(s):		Bahroun NHO; Perry JD; Stanforth SP; Dean JR;
Address:		"Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK. Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK. Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK. Electronic address: John.Dean@northumbria.ac.uk"
Journal Title:		Anal Chim Acta
Year:		2018
Volume:		20180418
Issue:
Page Number:		121 - 130
DOI:		10.1016/j.aca.2018.03.065
ISSN/ISBN:		1873-4324 (Electronic) 0003-2670 (Linking)
Abstract:		"Rapid, sensitive, and selective detection and identification of pathogenic bacteria is required in terms of food security. In this study, exogenous VOCs liberated by Salmonella strains have been identified and quantified via head space-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) in milk samples. The specific enzymes targeted for detection and/or differentiation of Salmonella were C8 esterase, alpha-galactosidase and pyrrolidonyl peptidase using the following enzyme substrates: 2-chlorophenyl octanoate, phenyl alpha-d-galactopyranoside and L-pyrrollidonyl fluoroanilide, respectively. Detection of the exogenous VOCs, 2-chlorophenol, phenol and 3-fluoraniline was possible with typical limits of detection of 0.014, 0.045 and 0.005?ª+mug/mL, respectively and correlation coefficients >0.99. The developed methodology was able to detect and identify Salmonella species within a 5?ª+h incubation at 37?ª+ degrees C by the detection of the liberated VOCs. It was found that the milk samples tested were Salmonella free"
Keywords:		Animals Food Contamination/analysis Gas Chromatography-Mass Spectrometry Milk/*microbiology Salmonella/*isolation & purification/*metabolism Solid Phase Microextraction Volatile Organic Compounds/*analysis/chemistry/isolation & purification/metabolism Enz;
Notes:		"MedlineBahroun, Najat H O Perry, John D Stanforth, Stephen P Dean, John R eng Netherlands 2018/06/10 Anal Chim Acta. 2018 Oct 22; 1028:121-130. doi: 10.1016/j.aca.2018.03.065. Epub 2018 Apr 18"