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J Biol Chem

Title:		Glc7p protein phosphatase inhibits expression of glutamine-fructose-6-phosphate transaminase from GFA1
Author(s):		Zheng J; Khalil M; Cannon JF;
Address:		"Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, Missouri 65212, USA"
Journal Title:		J Biol Chem
Year:		2000
Volume:		275
Issue:		24
Page Number:		18070 - 18078
DOI:		10.1074/jbc.M000918200
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Inhibitor-1 (I-1) is a specific inhibitor of protein phosphatase-1 (PP1). We assayed the ability of I-1 to inhibit Saccharomyces cerevisiae PP1, Glc7p, in vivo. Glc7p like other PP1 catalytic subunits associates with a variety of noncatalytic subunits, and Glc7p holoenzymes perform distinct physiological roles. Our results show that I-1 inhibits Glc7p holoenzymes that regulate transcription and mitosis, but holoenzymes responsible for meiosis and glycogen metabolism were unaffected. Additionally, we exploited a genetic screen for mutants that were dependent on I-1 to grow. This scheme can identify processes that are negatively regulated by Glc7p-catalyzed dephosphorylation. In this paper I-1-dependent gfa1 mutations were analyzed in detail. GFA1 encodes glutamine-fructose-6-phosphate transaminase. One or more phosphorylated proteins activate GFA1 transcription because the pheromone response and Pkc1p/mitogen-activated protein kinase pathways positively regulate GFA1 transcription. Our findings show that an I-1-sensitive Glc7p holoenzyme reduces GFA1 transcription. Therefore, GFA1 is a member of a growing list of genes that are negatively regulated by Glc7p dephosphorylation"
Keywords:		"Catalysis Fungal Proteins/genetics/*metabolism Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/genetics/*metabolism Mutagenesis, Site-Directed Phosphoprotein Phosphatases/*metabolism Phosphorylation Plasmids Protein Phosphatase 1 Saccharomyces c;"
Notes:		"MedlineZheng, J Khalil, M Cannon, J F eng AI07276/AI/NIAID NIH HHS/ GM40326/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2000/04/15 J Biol Chem. 2000 Jun 16; 275(24):18070-8. doi: 10.1074/jbc.M000918200"