| Title: | "MADS-box transcription factor Mcm1 controls cell cycle, fungal development, cell integrity and virulence in the filamentous insect pathogenic fungus Beauveria bassiana" |
| Author(s): | Zhao X; Yang X; Lu Z; Wang H; He Z; Zhou G; Luo Z; Zhang Y; |
| Address: | "Biotechnology Research Center, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, People's Republic of China" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "MADS-box transcription factor Mcm1 plays crucial roles in regulating mating processes and pathogenesis in some fungi. However, its roles are varied in fungal species, and its function remains unclear in entomopathogenic fungi. Here, Mcm1 orthologue, Bbmcm1, was characterized in a filamentous entomopathogenic fungus, Beauveria bassiana. Disruption of Bbmcm1 resulted in a distinct reduction in growth with abnormal conidiogenesis, and a significant decrease in conidial viability with abnormal germination. DeltaBbmcm1 displayed impaired cell integrity, with distorted cell wall structure and altered cell wall component. Abnormal cell cycle was detected in DeltaBbmcm1 with longer G(2) /M phase but shorter G(1) /G(0) and S phases in unicellular blastospores, and sparser septa in multicellular hyphae, which might be responsible for defects in development and differentiation due to the regulation of cell cycle-involved genes, as well as the corresponding cellular events-associated genes. Dramatically decreased virulence was examined in DeltaBbmcm1, with impaired ability to escape haemocyte encapsulation, which was consistent with markedly reduced cuticle-degrading enzyme production by repressing their coding genes, and downregulated fungal effector protein-coding genes, suggesting a novel role of Mcm1 in interaction with host insect. These data indicate that Mcm1 is a crucial regulator of development, cell integrity, cell cycle and virulence in insect fungal pathogens" |
| Notes: | "PubMed-not-MEDLINEZhao, Xin Yang, Xingju Lu, Zhuoyue Wang, Huifang He, Zhangjiang Zhou, Guangyan Luo, Zhibing Zhang, Yongjun eng cstc2017jcyjBX0067/Chongqing Research Program of Basic Research and Frontier Technology/ 2017YFD0200400/National Key R&D Program of China/ Karlsruhe Institute of Technology/ England 2019/04/12 Environ Microbiol. 2019 Sep; 21(9):3392-3416. doi: 10.1111/1462-2920.14629. Epub 2019 Apr 26" |
