| Title: | Molecular and Biochemical Characterization of Jasmonic Acid Carboxyl Methyltransferase Involved in Aroma Compound Production of Methyl Jasmonate during Black Tea Processing |
| Author(s): | Zhang J; Jia H; Zhu B; Li J; Yang T; Zhang ZZ; Deng WW; |
| Address: | "State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, People's Republic of China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Methyl jasmonate (MeJA), a volatile organic compound, is a principal flowery aromatic compound in tea. During the processing of black tea, MeJA is produced by jasmonic acid carboxyl methyltransferase (JMT) of the jasmonic acid (JA) substrate, forming a specific floral fragrance. CsJMT was cloned from tea leaves; the three-dimensional structure of CsJMT was predicted. Enzyme activity was identified, and protein purification was investigated. Site-directed deletions revealed that N-10, S-22, and Q-25 residues in the beginning amino acids played a key functional role in enzyme activity. The expression patterns of CsJMT in tea organs differed; the highest expression of CsJMT was observed in the fermentation process of black tea. These results aid in further understanding the synthesis of MeJA during black tea processing, which is crucial for improving black tea quality using specific fragrances and could be applied to the aromatic compound regulation and tea breeding improvement in further studies" |
| Keywords: | Acetates Cyclopentanes Methyltransferases *Odorants Oxylipins Plant Breeding *Tea CsJMT Ja MeJA black tea characterization; |
| Notes: | "MedlineZhang, Jing Jia, Huiyan Zhu, Biying Li, Junyao Yang, Tianyuan Zhang, Zheng-Zhu Deng, Wei-Wei eng 2021/03/06 J Agric Food Chem. 2021 Mar 17; 69(10):3154-3164. doi: 10.1021/acs.jafc.0c06248. Epub 2021 Mar 5" |
