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Genes Dev

Title:		Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins
Author(s):		Yuan YO; Stroke IL; Fields S;
Address:		"Department of Microbiology, State University of New York, Stony Brook 11794-5222"
Journal Title:		Genes Dev
Year:		1993
Volume:		7
Issue:		8
Page Number:		1584 - 1597
DOI:		10.1101/gad.7.8.1584
ISSN/ISBN:		0890-9369 (Print) 0890-9369 (Linking)
Abstract:		"In Saccharomyces cerevisiae, the STE12 protein mediates transcriptional induction of cell type-specific genes in response to pheromones. STE12 binds in vitro to the pheromone response elements (PREs) present in the control region of a-specific genes. STE12 is also required for transcription of alpha-specific genes, but there is no evidence that it binds directly to these genes. Instead, the MAT alpha-encoded protein alpha 1 and the MCM1 product bind to the DNA element that is responsible for alpha-specific and a-factor-inducible expression. To explore the role of STE12 in the pheromone induction of alpha-specific genes, we cloned STE12 and MAT alpha 1 homologs from the related yeast Kluyveromyces lactis. The K. lactis STE12 protein did not cooperate with the S. cerevisiae alpha 1 protein to promote the overall mating process or the induction of transcription of an alpha-specific gene. However, introduction of both K. lactis STE12 along with K. lactis alpha 1 did restore mating, suggesting that an interaction between STE12 and alpha 1 is important for alpha-specific gene activation. We also show that bacterially expressed STE12 and alpha 1 are able to form a complex in vitro. Thus, we demonstrate a coupling in alpha cells between a protein functioning in cell identity, alpha 1, with a protein responsive to the pheromone-induced signal STE12"
Keywords:		"Amino Acid Sequence Base Sequence Cell Differentiation/genetics Conserved Sequence DNA-Binding Proteins/physiology Fungal Proteins/chemistry/*genetics/*metabolism GTP-Binding Proteins/physiology *Gene Expression Regulation, Fungal Kluyveromyces/genetics M;"
Notes:		"MedlineYuan, Y O Stroke, I L Fields, S eng 5 T32 CA09176/CA/NCI NIH HHS/ GM49065/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1993/08/01 Genes Dev. 1993 Aug; 7(8):1584-97. doi: 10.1101/gad.7.8.1584"