« Previous Abstract[Competition and allelopathy of rice isogenic lines having similar genetic background but different plant morphology against weed]    Next Abstract"Characterization of TRPC2, an essential genetic component of VNS chemoreception, provides insights into the evolution of pheromonal olfaction in secondary-adapted marine mammals" »

Mol Biol Cell

Title:		Counteractive control of polarized morphogenesis during mating by mitogen-activated protein kinase Fus3 and G1 cyclin-dependent kinase
Author(s):		Yu L; Qi M; Sheff MA; Elion EA;
Address:		"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115-5730, USA"
Journal Title:		Mol Biol Cell
Year:		2008
Volume:		20080206
Issue:		4
Page Number:		1739 - 1752
DOI:		10.1091/mbc.e07-08-0757
ISSN/ISBN:		1939-4586 (Electronic) 1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"Cell polarization in response to external cues is critical to many eukaryotic cells. During pheromone-induced mating in Saccharomyces cerevisiae, the mitogen-activated protein kinase (MAPK) Fus3 induces polarization of the actin cytoskeleton toward a landmark generated by the pheromone receptor. Here, we analyze the role of Fus3 activation and cell cycle arrest in mating morphogenesis. The MAPK scaffold Ste5 is initially recruited to the plasma membrane in random patches that polarize before shmoo emergence. Polarized localization of Ste5 is important for shmooing. In fus3 mutants, Ste5 is recruited to significantly more of the plasma membrane, whereas recruitment of Bni1 formin, Cdc24 guanine exchange factor, and Ste20 p21-activated protein kinase are inhibited. In contrast, polarized recruitment still occurs in a far1 mutant that is also defective in G1 arrest. Remarkably, loss of Cln2 or Cdc28 cyclin-dependent kinase restores polarized localization of Bni1, Ste5, and Ste20 to a fus3 mutant. These and other findings suggest Fus3 induces polarized growth in G1 phase cells by down-regulating Ste5 recruitment and by inhibiting Cln/Cdc28 kinase, which prevents basal recruitment of Ste5, Cdc42-mediated asymmetry, and mating morphogenesis"
Keywords:		"Actins/metabolism Adaptor Proteins, Signal Transducing/genetics/metabolism CDC28 Protein Kinase, S cerevisiae/genetics/metabolism Cell Cycle Proteins/genetics/metabolism Cell Membrane/metabolism Cell Polarity Cyclin-Dependent Kinases/genetics/*metabolism;"
Notes:		"MedlineYu, Lu Qi, Maosong Sheff, Mark A Elion, Elaine A eng NIGMSRO1 46962/PHS HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2008/02/08 Mol Biol Cell. 2008 Apr; 19(4):1739-52. doi: 10.1091/mbc.e07-08-0757. Epub 2008 Feb 6"