« Previous AbstractAnalysis of volatile fatty acids in wastewater collected from a pig farm by a solid phase microextraction method    Next AbstractMolecular evolutionary characterization of a V1R subfamily unique to strepsirrhine primates »

BMC Genomics

Title:		"Expressed sequences tags of the anther smut fungus, Microbotryum violaceum, identify mating and pathogenicity genes"
Author(s):		Yockteng R; Marthey S; Chiapello H; Gendrault A; Hood ME; Rodolphe F; Devier B; Wincker P; Dossat C; Giraud T;
Address:		"UMR 8079 CNRS-UPS, Ecologie, Systematique et Evolution, Batiment 360, Universite Paris-Sud, F-91405 Orsay Cedex, France. yockteng@mnhn.fr"
Journal Title:		BMC Genomics
Year:		2007
Volume:		20070810
Issue:
Page Number:		272 -
DOI:		10.1186/1471-2164-8-272
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: The basidiomycete fungus Microbotryum violaceum is responsible for the anther-smut disease in many plants of the Caryophyllaceae family and is a model in genetics and evolutionary biology. Infection is initiated by dikaryotic hyphae produced after the conjugation of two haploid sporidia of opposite mating type. This study describes M. violaceum ESTs corresponding to nuclear genes expressed during conjugation and early hyphal production. RESULTS: A normalized cDNA library generated 24,128 sequences, which were assembled into 7,765 unique genes; 25.2% of them displayed significant similarity to annotated proteins from other organisms, 74.3% a weak similarity to the same set of known proteins, and 0.5% were orphans. We identified putative pheromone receptors and genes that in other fungi are involved in the mating process. We also identified many sequences similar to genes known to be involved in pathogenicity in other fungi. The M. violaceum EST database, MICROBASE, is available on the Web and provides access to the sequences, assembled contigs, annotations and programs to compare similarities against MICROBASE. CONCLUSION: This study provides a basis for cloning the mating type locus, for further investigation of pathogenicity genes in the anther smut fungi, and for comparative genomics"
Keywords:		"Base Sequence DNA, Fungal Databases, Nucleic Acid *Expressed Sequence Tags Fungi Gene Library Genes, Fungal/*genetics Genes, Mating Type, Fungal/*genetics Plant Diseases/microbiology Virulence/*genetics;"
Notes:		"MedlineYockteng, Roxana Marthey, Sylvain Chiapello, Helene Gendrault, Annie Hood, Michael E Rodolphe, Francois Devier, Benjamin Wincker, Patrick Dossat, Carole Giraud, Tatiana eng Research Support, Non-U.S. Gov't England 2007/08/19 BMC Genomics. 2007 Aug 10; 8:272. doi: 10.1186/1471-2164-8-272"