| Title: | Multivariate analysis of the volatile components in tobacco based on infrared-assisted extraction coupled to headspace solid-phase microextraction and gas chromatography-mass spectrometry |
| Author(s): | Yang Y; Pan Y; Zhou G; Chu G; Jiang J; Yuan K; Xia Q; Cheng C; |
| Address: | "Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China. Department of Chemistry, Zhejiang University, Hangzhou, P. R. China" |
| ISSN/ISBN: | 1615-9314 (Electronic) 1615-9306 (Linking) |
| Abstract: | "A novel infrared-assisted extraction coupled to headspace solid-phase microextraction followed by gas chromatography with mass spectrometry method has been developed for the rapid determination of the volatile components in tobacco. The optimal extraction conditions for maximizing the extraction efficiency were as follows: 65 mum polydimethylsiloxane-divinylbenzene fiber, extraction time of 20 min, infrared power of 175 W, and distance between the infrared lamp and the headspace vial of 2 cm. Under the optimum conditions, 50 components were found to exist in all ten tobacco samples from different geographical origins. Compared with conventional water-bath heating and nonheating extraction methods, the extraction efficiency of infrared-assisted extraction was greatly improved. Furthermore, multivariate analysis including principal component analysis, hierarchical cluster analysis, and similarity analysis were performed to evaluate the chemical information of these samples and divided them into three classifications, including rich, moderate, and fresh flavors. The above-mentioned classification results were consistent with the sensory evaluation, which was pivotal and meaningful for tobacco discrimination. As a simple, fast, cost-effective, and highly efficient method, the infrared-assisted extraction coupled to headspace solid-phase microextraction technique is powerful and promising for distinguishing the geographical origins of the tobacco samples coupled to suitable chemometrics" |
| Keywords: | Dimethylpolysiloxanes Gas Chromatography-Mass Spectrometry Multivariate Analysis Phytochemicals/*analysis Polyvinyls Solid Phase Microextraction Tobacco/*chemistry Volatile Organic Compounds/*analysis Chemometrics Gas chromatography with mass spectrometry; |
| Notes: | "MedlineYang, Yanqin Pan, Yuanjiang Zhou, Guojun Chu, Guohai Jiang, Jian Yuan, Kailong Xia, Qian Cheng, Changhe eng Germany 2016/09/20 J Sep Sci. 2016 Nov; 39(21):4192-4201. doi: 10.1002/jssc.201600503. Epub 2016 Oct 10" |
