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J Fungi (Basel)

Title:		Antifungal Activities of Volatile Secondary Metabolites of Four Diaporthe Strains Isolated from Catharanthus roseus
Author(s):		Yan DH; Song X; Li H; Luo T; Dou G; Strobel G;
Address:		"Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Hai Dian District, Beijing 100091, China. yandh@caf.ac.cn. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Hai Dian District, Beijing 100091, China. songxiaoyucaf@gmail.com. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Hai Dian District, Beijing 100091, China. lhc1994224@163.com. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Tianhe District, Guangzhou 510520, China. luots@126.com. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Hai Dian District, Beijing 100091, China. dgmgogogo@126.com. Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA. uplgs@montana.edu"
Journal Title:		J Fungi (Basel)
Year:		2018
Volume:		20180530
Issue:		2
Page Number:		-
DOI:		10.3390/jof4020065
ISSN/ISBN:		2309-608X (Electronic) 2309-608X (Linking)
Abstract:		"Four endophytic fungi were isolated from the medicinal plant, Catharanthus roseus, and were identified as Diaporthe spp. with partial translation elongation factor 1-alpha (TEF1), beta-tubulin (TUB), histone H3 (HIS), calmodulin (CAL) genes, and rDNA internal transcribed spacer (ITS) region (TEF1-TUB-HIS--CAL-ITS) multigene phylogeny suggested for species delimitation in the Diaporthe genus. Each fungus produces a unique mixture of volatile organic compounds (VOCs) with an abundant mixture of terpenoids analyzed by headspace solid-phase microextraction (SPME) fiber-GC/MS. These tentatively-detected terpenes included alpha-muurolene, beta-phellandrene, gamma-terpinene, and alpha-thujene, as well as other minor terpenoids, including caryophyllene, patchoulene, cedrene, 2-carene, and thujone. The volatile metabolites of each isolate showed antifungal properties against a wide range of plant pathogenic test fungi and oomycetes, including Alternaria alternata, Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium graminearum, and Phytophthora cinnamomi. The growth inhibition of the pathogens varied between 10% and 60% within 72 h of exposure. To our knowledge, the endophytic Diaporthe-like strains are first reported from Catharanthus roseus. VOCs produced by each strain of the endophytic Diaporthe fungi were unique components with dominant monoterpenes comparing to known Diaporthe fungal VOCs. A discussion is presented on the inhibitive bioactivities of secondary metabolites among endophytic Diaporthe fungi and this medicinal plant"
Keywords:		Catharanthus roseus Diaporthe spp.antifungal bioactivity endophytic fungi inhibition pathogens terpene volatile organic compounds (VOCs);
Notes:		"PubMed-not-MEDLINEYan, Dong-Hui Song, Xiaoyu Li, Hongchang Luo, Tushou Dou, Guiming Strobel, Gary eng Switzerland 2018/06/01 J Fungi (Basel). 2018 May 30; 4(2):65. doi: 10.3390/jof4020065"