| Title: | Isolation of anti-algal substances from Cylindrotheca closterium and their inhibition activity on bloom-forming Prorocentrum donghaiense |
| Author(s): | Xu W; Tan L; Guo X; Wang J; |
| Address: | "Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China. Electronic address: lijutan@ouc.edu.cn. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China. Electronic address: jtwang@ouc.edu.cn" |
| DOI: | 10.1016/j.ecoenv.2020.110180 |
| ISSN/ISBN: | 1090-2414 (Electronic) 0147-6513 (Linking) |
| Abstract: | "This paper studied the allelopathic effect of Cylindrotheca closterium on the growth of Prorocentrum donghaiense, the model of harmful algal blooms in aquatic environment, by the co-culture tests and bioassay-guided fraction methods. The growth of P. donghaiense in co-cultures was observably suppressed by C. closterium, and P. donghaiense biomass in C/P = 3: 1 group increased slowly with a low growth rate of 0.18 d(-1) after 4 days. Petroleum ether (PE) extract derived from C. closterium filtrates was isolated by C18 column and the allelopathy of all isolated fractions for P. donghaiense was investigated. After 96 h cultivation, the inhibition ratio of PE-?a= and PE-?aª fractions on P. donghaiense could reach up to 70.2% and 64.3% at the concentration of 10-fold when compared to control, while the other fractions displayed relatively low inhibitory effects on P. donghaiense. PE-?a= and PE-?aª fractions also decreased the chlorophyll content and maximum quantum yield of photosystem II (Fv/Fm) of P. donghaiense cells. The activities of superoxide dismutase (SOD), one of antioxidant enzymes, reduced around 8.3% and 13.7% following exposure to 2-fold PE-?a= and PE-?aª, and was significantly decreased following higher exposure concentrations. After 96 h of 10-fold PE-?a= and PE-?aª treatments, Catalase (CAT) activity reduced to 44.86% and 46.42% of that observed in the control group. At the same time, a significant increase in malondialdehyde (MDA) contents was observed. These findings suggested that PE-?a= and PE-?aª fractions contained main allelochemicals and possibly acted as promising algistatic agents for emergency handling of P. donghaiense blooms" |
| Keywords: | Allelopathy Biomass Chlorophyll/metabolism Diatoms/*chemistry Dinoflagellida/*drug effects/growth & development Harmful Algal Bloom/*drug effects Herbicides/isolation & purification/*pharmacology Pheromones/isolation & purification/*pharmacology Photosyst; |
| Notes: | "MedlineXu, Wenjing Tan, Liju Guo, Xin Wang, Jiangtao eng Netherlands 2020/01/14 Ecotoxicol Environ Saf. 2020 Mar 1; 190:110180. doi: 10.1016/j.ecoenv.2020.110180. Epub 2020 Jan 8" |
