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Neuron

Title:		Drosophila OBP LUSH is required for activity of pheromone-sensitive neurons
Author(s):		Xu P; Atkinson R; Jones DN; Smith DP;
Address:		"Department of Pharmacology, Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75335, USA"
Journal Title:		Neuron
Year:		2005
Volume:		45
Issue:		2
Page Number:		193 - 200
DOI:		10.1016/j.neuron.2004.12.031
ISSN/ISBN:		0896-6273 (Print) 0896-6273 (Linking)
Abstract:		"Odorant binding proteins (OBPs) are extracellular proteins localized to the chemosensory systems of most terrestrial species. OBPs are expressed by nonneuronal cells and secreted into the fluid bathing olfactory neuron dendrites. Several members have been shown to interact directly with odorants, but the significance of this is not clear. We show that the Drosophila OBP lush is completely devoid of evoked activity to the pheromone 11-cis vaccenyl acetate (VA), revealing that this binding protein is absolutely required for activation of pheromone-sensitive chemosensory neurons. lush mutants are also defective for pheromone-evoked behavior. Importantly, we identify a genetic interaction between lush and spontaneous activity in VA-sensitive neurons in the absence of pheromone. The defects in spontaneous activity and VA sensitivity are reversed by germline transformation with a lush transgene or by introducing recombinant LUSH protein into mutant sensilla. These studies directly link pheromone-induced behavior with OBP-dependent activation of a subset of olfactory neurons"
Keywords:		Action Potentials/drug effects/physiology Alcohols/pharmacology Animals Cell Differentiation/physiology Cell Lineage/drug effects/physiology Drosophila melanogaster Germ-Line Mutation/genetics Oleic Acids/pharmacology Olfactory Pathways/drug effects/*phys;neuroscience;
Notes:		"MedlineXu, Pingxi Atkinson, Rachel Jones, David N M Smith, Dean P eng R01 AA013618/AA/NIAAA NIH HHS/ R01 AA013618-05/AA/NIAAA NIH HHS/ R01 DC002539/DC/NIDCD NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2005/01/25 Neuron. 2005 Jan 20; 45(2):193-200. doi: 10.1016/j.neuron.2004.12.031"