| Title: | Increased apoptosis during neonatal brain development underlies the adult behavioral deficits seen in mice lacking a functional paternally expressed gene 3 (Peg3) |
| Author(s): | Broad KD; Curley JP; Keverne EB; |
| Address: | "Sub-Department of Animal Behaviour, University of Cambridge, Madingley, Cambridge, CB3 8AA, United Kingdom. kdb1000@cam.ac.uk" |
| ISSN/ISBN: | 1932-8451 (Print) 1932-8451 (Linking) |
| Abstract: | "Inactivation of the maternally imprinted, paternally expressed gene 3 (Peg3) induces deficits in olfactory function, sexual and maternal behaviors, oxytocin neuron number, metabolic homeostasis and growth. Peg3 is expressed in a number of developing hypothalamic and basal forebrain structures and is a component of the P53 apoptosis pathway. Peg3 inactivation in neuronal cell culture lines inhibits P53 mediated apoptosis, which is important in the early postnatal development and sexual differentiation of the brain. In this study, we investigated the effect of inactivating the Peg3 gene on the incidence of caspase 3 positive cells (a marker of apoptosis) in 4- and 6-day postpartum mouse brain. Inactivating the Peg3 gene resulted in an increase in the incidence of total forebrain caspase 3 positive cells at 4 and 6 days postpartum. Increases in specific neuroanatomical regions including the bed nucleus of the stria terminalis, nucleus accumbens, caudate putamen, medial pre-optic area, arcuate nucleus, medial amygdala, anterior cortical and posteriodorsal amygdaloid nuclei, were also observed. In wild-type mice, sex differences in the incidence of caspase 3 positive cells in the medial amygdala, bed nucleus of the stria terminalis, nucleus accumbens, arcuate nucleus and the M2 motor cortex, were also observed. This neural sex difference was ameliorated in the Peg-3 mutant. These findings suggest that the neuronal and behavioral deficits seen in mice lacking a functional Peg3 gene are mediated by increases in the incidence of early neonatal apoptosis in neuroanatomical regions important for reproductive behavior, olfactory and pheromonal processing, thermoregulation and reward" |
| Keywords: | "Age Factors Animals Animals, Newborn Apoptosis/*genetics Brain/anatomy & histology/*growth & development/metabolism Caspase 3/metabolism Female Gene Expression Regulation, Developmental/*genetics Kruppel-Like Transcription Factors/*genetics/metabolism Mal;" |
| Notes: | "MedlineBroad, Kevin D Curley, James P Keverne, Eric B eng 2009/02/19 Dev Neurobiol. 2009 Apr; 69(5):314-25. doi: 10.1002/dneu.20702" |
