| Title: | The G(1) cyclin Cln3 promotes cell cycle entry via the transcription factor Swi6 |
| Author(s): | Wijnen H; Landman A; Futcher B; |
| Address: | "Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA" |
| DOI: | 10.1128/MCB.22.12.4402-4418.2002 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G(1) is promoted by the G(1) cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G(1) phase, including control of cell size, pheromone sensitivity, cell cycle progress, and transcription, require the protein Swi6. Swi6 is a component of two related transcription factors, SBF and MBF, which are known to regulate many genes at the G(1)-S transition. The Cln3-Cdc28 complex somehow activates SBF and MBF, but there was no evidence for direct phosphorylation of SBF/MBF by Cln3-Cdc28 or for a stable complex between SBF/MBF and Cln3-Cdc28. The activation also does not depend on the ability of Cln3 to activate transcription when artificially recruited directly to a promoter. The amino terminus and the leucine zipper of Swi6 are important for the ability of Swi6 to respond to Cln3 but are not essential for the basal transcriptional activity of Swi6. Cln3-Cdc28 may activate SBF and MBF indirectly, perhaps by phosphorylating some intermediary protein" |
| Keywords: | "CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle/*genetics Cyclins/genetics/*metabolism DNA-Binding Proteins Fungal Proteins/genetics/*metabolism G1 Phase/genetics Mutation Pheromones/metabolism Phosphorylation Saccharomyces cerevisiae Proteins/ge;" |
| Notes: | "MedlineWijnen, Herman Landman, Allison Futcher, Bruce eng R01 GM039978/GM/NIGMS NIH HHS/ R01 GM39978/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2002/05/25 Mol Cell Biol. 2002 Jun; 22(12):4402-18. doi: 10.1128/MCB.22.12.4402-4418.2002" |
