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Environ Microbiol

Title:		A new dimethylsulfoniopropionate lyase of the cupin superfamily in marine bacteria
Author(s):		Wang SY; Zhang N; Teng ZJ; Wang XD; Todd JD; Zhang YZ; Cao HY; Li CY;
Address:		"Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, Qingdao, China. State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China. School of Biological Sciences, University of East Anglia, Norwich, UK"
Journal Title:		Environ Microbiol
Year:		2023
Volume:		20230228
Issue:		7
Page Number:		1238 - 1249
DOI:		10.1111/1462-2920.16355
ISSN/ISBN:		1462-2920 (Electronic) 1462-2912 (Linking)
Abstract:		"Dimethylsulfoniopropionate (DMSP) is a marine organosulfur compound with important roles in stress protection, marine biogeochemical cycling, chemical signalling and atmospheric chemistry. Diverse marine microorganisms catabolize DMSP via DMSP lyases to generate the climate-cooling gas and info-chemical dimethyl sulphide. Abundant marine heterotrophs of the Roseobacter group (MRG) are well known for their ability to catabolize DMSP via diverse DMSP lyases. Here, a new DMSP lyase DddU within the MRG strain Amylibacter cionae H-12 and other related bacteria was identified. DddU is a cupin superfamily DMSP lyase like DddL, DddQ, DddW, DddK and DddY, but shares <15% amino acid sequence identity with these enzymes. Moreover, DddU proteins forms a distinct clade from these other cupin-containing DMSP lyases. Structural prediction and mutational analyses suggested that a conserved tyrosine residue is the key catalytic amino acid residue in DddU. Bioinformatic analysis indicated that the dddU gene, mainly from Alphaproteobacteria, is widely distributed in the Atlantic, Pacific, Indian and polar oceans. For reference, dddU is less abundant than dddP, dddQ and dddK, but much more frequent than dddW, dddY and dddL in marine environments. This study broadens our knowledge on the diversity of DMSP lyases, and enhances our understanding of marine DMSP biotransformation"
Keywords:		Amino Acid Sequence *Carbon-Sulfur Lyases/genetics/metabolism Oceans and Seas *Sulfonium Compounds/metabolism Sulfides/metabolism;
Notes:		"MedlineWang, Shu-Yan Zhang, Nan Teng, Zhao-Jie Wang, Xiao-Di Todd, Jonathan D Zhang, Yu-Zhong Cao, Hai-Yan Li, Chun-Yang eng BB/X005968/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2023/02/23 Environ Microbiol. 2023 Jul; 25(7):1238-1249. doi: 10.1111/1462-2920.16355. Epub 2023 Feb 28"