| Title: | CRISPR/Cas9-mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella |
| Author(s): | Wang D; Chen J; Yuan Y; Yu L; Yang G; Chen W; |
| Address: | "State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China. Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, China. Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China. Key Laboratory of Green Control of Insect Pests, (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, China. Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou, China. Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou, China" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Linking) |
| Abstract: | "Circadian clocks control the rhythmicity of many behaviors and physiological features of insects. To study the circadian clock of the moth Plutella xylostella, we employed CRISPR/Cas9-mediated genome editing to investigate the effect of loss of the clock gene period on the circadian rhythms. P. xylostella harbors a single copy of period. Phylogenetic analysis showed that P. xylostella PERIOD is more homologous to mouse PERIOD than the PERIOD proteins from bees, flies, mosquitos, and many other Lepidoptera, such as Danaus plexippus and Bombyx mori. The circadian rhythms in adult locomotor activity were altered in the period knockout strain of P. xylostella under light-dark (LD) and continuous dark (DD) conditions. Under the LD cycle, the wild-type moths displayed nocturnal activity with activity peaking very early after lights off and quickly declining after lights on. In contrast, the period knockout strain had no peak in activity when the lights were turned off and exhibited steady activity throughout the hours of darkness. Interestingly, under DD conditions, our results showed that the locomotor rhythm can be maintained without period gene, but at a lower rhythmicity ratio than wild-type. In addition, knockout of period in P. xylostella changed circadian rhythms patterns related to pupal eclosion, mating, egg-laying, and egg hatching. Mechanistically, loss of PERIOD disrupted the molecular rhythm of period and changed the clock transcription rhythm in the heads of the moths under LD and DD conditions. Together, our study indicates that the PERIOD is required for normal expression of many behavioral rhythms in P. xylostella" |
| Keywords: | Animals Mice *Moths/genetics CRISPR-Cas Systems Phylogeny Circadian Rhythm/genetics Photoperiod CRISPR/Cas9 Plutella xylostella circadian rhythm period; |
| Notes: | "MedlineWang, Danfeng Chen, Jing Yuan, Yao Yu, Lingqi Yang, Guang Chen, Wenfeng eng 31772237/National Natural Science Foundation of China/ 31970461/National Natural Science Foundation of China/ 2020J02027/Fujian Natural Science Foundation/ GXRC-20070/Qi-Shan scholar grant of Fuzhou University/ Australia 2022/11/16 Insect Sci. 2023 Jun; 30(3):637-649. doi: 10.1111/1744-7917.13139. Epub 2022 Nov 29" |
