| Title: | Characterization of a mitogen-activated protein kinase from Pneumocystis carinii |
| Author(s): | Thomas CF; Kottom TJ; Leof EB; Limper AH; |
| Address: | "Thoracic Diseases Research Unit, Division of Pulmonary, Critical Care and Internal Medicine, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA" |
| DOI: | 10.1152/ajplung.1998.275.1.L193 |
| ISSN/ISBN: | 0002-9513 (Print) 0002-9513 (Linking) |
| Abstract: | "The pathogenic fungus Pneumocystis carinii causes severe pneumonia in patients with impaired immunity, particularly patients with acquired immunodeficiency syndrome. The life cycle of P. carinii is poorly understood, and the inability to continuously culture P. carinii is a major limitation in understanding its cell biology. In fungi homologous to P. carinii, pheromone mating factors signal through a mitogen-activated protein kinase (MAPK) signal transduction cascade, resulting in mitotic cell cycle arrest and entry into a pathway of conjugation, cellular differentiation, and proliferation. Using degenerate PCR and library screening, we have identified a MAPK cDNA in P. carinii that is highly homologous to fungal MAPKs involved in the pheromone mating signal transduction cascade, and we demonstrate MAPK activity in P. carinii lysates with a specific antiserum derived from the translated P. carinii MAPK cDNA sequence" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases/chemistry/*genetics/*metabolism *Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Fungi/enzymology Gene Library Humans Kinetics Molecular Sequence Data Pneumoc;" |
| Notes: | "MedlineThomas, C F Jr Kottom, T J Leof, E B Limper, A H eng R01 HL062150/HL/NHLBI NIH HHS/ AI-34336-05/AI/NIAID NIH HHS/ HL-55934-03/HL/NHLBI NIH HHS/ HL-57125-01/HL/NHLBI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1998/08/05 Am J Physiol. 1998 Jul; 275(1):L193-9. doi: 10.1152/ajplung.1998.275.1.L193" |
