| Title: | "Immunoreactivities of AR, ERalpha, ERbeta and aromatase in the nuptial pad of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period" |
| Author(s): | Tang Z; Chen Y; Ren B; Wang X; Zhang H; Han Y; Yuan Z; Weng Q; |
| Address: | "Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. tangzeqi@bjfu.edu.cn. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. qiangweng@bjfu.edu.cn. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. 774575711@qq.com. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. wangyixin808@163.com. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. zhanghl0117@hotmail.com. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. thinkinghyy@bjfu.edu.cn. Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing. zryuan@bjfu.edu.cn" |
| ISSN/ISBN: | 2038-8306 (Electronic) 1121-760X (Print) 1121-760X (Linking) |
| Abstract: | "There is a prominent local raised pad called nuptial pad on the forelimb of Chinese brown frog (Rana dybowskii), which is hypothetically concluded as an enhancement of the grip and a spreader of pheromone during the amplexus. In this study, we investigated the immunolocalization and protein expression levels of AR, ERalpha, ERbeta and aromatase in the nuptial pad of R. dybowskii during pre-hibernation and the breeding period. Histologically, the annual development of the nuptial pad in R. dybowskii is manifested as the larger area of specialized mucous gland and the longer length of papillary epidermal projection during the breeding period. AR, ERalpha, ERbeta and aromatase are present in the stratum granulosum, stratum spinosum, stratum basale and the secretory portion of specialized mucous glands during both periods. Western blotting results confirmed that AR, ERalpha and ERbeta protein levels are higher during pre-hibernation than those during the breeding season. These results suggest that nuptial pad is the direct target organ of androgen and estrogen. Androgen may participate in the regulation of annual development and glandular function of nuptial pad, and estrogen may play an endocrine, autocrine or paracrine role during pre-hibernation and the breeding period" |
| Keywords: | "Animals Aromatase/*metabolism Breeding Estrogen Receptor alpha/*metabolism Estrogen Receptor beta/*metabolism Exocrine Glands/cytology/*metabolism Forelimb/cytology/metabolism Hibernation/physiology Immunohistochemistry Male Ranidae/*metabolism Receptors, ;" |
| Notes: | "MedlineTang, Zeqi Chen, Yuan Ren, Baolong Wang, Xi Zhang, Haolin Han, Yingying Yuan, Zhengrong Weng, Qiang eng Italy 2021/04/14 Eur J Histochem. 2021 Apr 13; 65(2):3206. doi: 10.4081/ejh.2021.3206" |
