| Title: | Non-native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stability |
| Author(s): | Studer SV; Schwartzman JA; Ho JS; Geske GD; Blackwell HE; Ruby EG; |
| Address: | "Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison WI 53706. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison WI 53706" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Print) 1462-2912 (Linking) |
| Abstract: | "Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR-type N-acyl L-homoserine (AHL) quorum sensing is common in Gram-negative Proteobacteria, and many members of this group have additional quorum-sensing networks. The bioluminescent symbiont Vibrio fischeri encodes two AHL signal synthases: AinS and LuxI. AinS-dependent quorum sensing converges with LuxI-dependent quorum sensing at the LuxR regulatory element. Both AinS- and LuxI-mediated signalling are required for efficient and persistent colonization of the squid host, Euprymna scolopes. The basis of the mutualism is symbiont bioluminescence, which is regulated by both LuxI- and AinS-dependent quorum sensing, and is essential for maintaining a colonization of the host. Here, we used chemical and genetic approaches to probe the dynamics of LuxI- and AinS-mediated regulation of bioluminescence during symbiosis. We demonstrate that both native AHLs and non-native AHL analogues can be used to non-invasively and specifically modulate induction of symbiotic bioluminescence via LuxI-dependent quorum sensing. Our data suggest that the first day of colonization, during which symbiont bioluminescence is induced by LuxIR, is a critical period that determines the stability of the V. fischeri population once symbiosis is established" |
| Keywords: | "4-Butyrolactone/*analogs & derivatives/metabolism Aliivibrio fischeri/*genetics/metabolism Animals Bacterial Proteins/*genetics/metabolism Decapodiformes/microbiology *Gene Expression Regulation, Bacterial Luminescence Quorum Sensing/*genetics Repressor P;" |
| Notes: | "MedlineStuder, Sarah V Schwartzman, Julia A Ho, Jessica S Geske, Grant D Blackwell, Helen E Ruby, Edward G eng R01 GM109403/GM/NIGMS NIH HHS/ R01 AI050661/AI/NIAID NIH HHS/ T32 GM008505/GM/NIGMS NIH HHS/ T32 GM007215/GM/NIGMS NIH HHS/ RR12294/OD11024/OD/NIH HHS/ T32 GM07215/GM/NIGMS NIH HHS/ AI050661/AI/NIAID NIH HHS/ R01 AI063326/AI/NIAID NIH HHS/ R01 OD011024/OD/NIH HHS/ R37 AI050661/AI/NIAID NIH HHS/ R01 RR012294/RR/NCRR NIH HHS/ R01 GM099507/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2013/11/07 Environ Microbiol. 2014 Aug; 16(8):2623-2634. doi: 10.1111/1462-2920.12322. Epub 2013 Nov 28" |
