| Title: | Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain |
| Author(s): | Sterne GR; Otsuna H; Dickson BJ; Scott K; |
| Address: | "University of California Berkeley, Berkeley, United States. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States. Queensland Brain Institute, University of Queensland, Queensland, Australia" |
| ISSN/ISBN: | 2050-084X (Electronic) 2050-084X (Linking) |
| Abstract: | "Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and move away from threats. In insects, the subesophageal zone (SEZ) is a brain region that receives gustatory, pheromonal, and mechanosensory inputs and contributes to the control of diverse behaviors, including feeding, grooming, and locomotion. Despite its importance in sensorimotor transformations, the study of SEZ circuits has been hindered by limited knowledge of the underlying diversity of SEZ neurons. Here, we generate a collection of split-GAL4 lines that provides precise genetic targeting of 138 different SEZ cell types in adult Drosophila melanogaster, comprising approximately one third of all SEZ neurons. We characterize the single-cell anatomy of these neurons and find that they cluster by morphology into six supergroups that organize the SEZ into discrete anatomical domains. We find that the majority of local SEZ interneurons are not classically polarized, suggesting rich local processing, whereas SEZ projection neurons tend to be classically polarized, conveying information to a limited number of higher brain regions. This study provides insight into the anatomical organization of the SEZ and generates resources that will facilitate further study of SEZ neurons and their contributions to sensory processing and behavior" |
| Keywords: | Animals Cell Line Cluster Analysis *Drosophila melanogaster/genetics/physiology Female *Motor Cortex/cytology/physiology *Neurons/cytology/physiology *Taste Perception/genetics/physiology D.melanogaster anatomy brain neuroscience split-GAL4 subesophageal; |
| Notes: | "MedlineSterne, Gabriella R Otsuna, Hideo Dickson, Barry J Scott, Kristin eng F32 DK117671/DK/NIDDK NIH HHS/ R01 NS110060/NS/NINDS NIH HHS/ HHMI/Howard Hughes Medical Institute/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2021/09/03 Elife. 2021 Sep 2; 10:e71679. doi: 10.7554/eLife.71679" |
