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Insect Biochem Mol Biol

Title:		Functional characterization of myrcene hydroxylases from two geographically distinct Ips pini populations
Author(s):		Song M; Kim AC; Gorzalski AJ; MacLean M; Young S; Ginzel MD; Blomquist GJ; Tittiger C;
Address:		"Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA"
Journal Title:		Insect Biochem Mol Biol
Year:		2013
Volume:		20130131
Issue:		4
Page Number:		336 - 343
DOI:		10.1016/j.ibmb.2013.01.003
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Ips pini bark beetles use myrcene hydroxylases to produce the aggregation pheromone component, ipsdienol, from myrcene. The enantiomeric ratio of pheromonal ipsdienol is an important prezygotic mating isolation mechanism of I. pini and differs among geographically distinct populations. We explored the substrate and product ranges of myrcene hydroxylases (CYP9T2 and CYP9T3) from reproductively-isolated western and eastern I. pini. The two cytochromes P450 share 94% amino acid identity. CYP9T2 mRNA levels were not induced in adults exposed to myrcene-saturated atmosphere. Functional assays of recombinant enzymes showed both hydroxylated myrcene, (+)- and (-)-alpha-pinene, 3-carene, and R-(+)-limonene, but not alpha-phellandrene, (-)-beta-pinene, gamma-terpinene, or terpinolene, with evidence that CYP9T2 strongly preferred myrcene over other substrates. They differed in the enantiomeric ratios of ipsdienol produced from myrcene, and in the products resulting from different alpha-pinene enantiomers. These data provide new information regarding bark beetle pheromone evolution and factors affecting cytochrome P450 structure-function relationships"
Keywords:		Acyclic Monoterpenes Alkenes/*metabolism Amino Acid Sequence Animals Coleoptera/chemistry/*enzymology/genetics Female Geography Insect Proteins/chemistry/genetics/*metabolism Male Mixed Function Oxygenases/chemistry/genetics/*metabolism Molecular Sequence;
Notes:		"MedlineSong, Minmin Kim, Amy C Gorzalski, Andrew J MacLean, Marina Young, Sharon Ginzel, Matthew D Blomquist, Gary J Tittiger, Claus eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2013/02/05 Insect Biochem Mol Biol. 2013 Apr; 43(4):336-43. doi: 10.1016/j.ibmb.2013.01.003. Epub 2013 Jan 31"