| Title: | Effects of Volatile Organic Compounds on Biofilms and Swimming Motility of Agrobacterium tumefaciens |
| Author(s): | Sidorova DE; Skripka MI; Khmel IA; Koksharova OA; Plyuta VA; |
| Address: | "Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182 Moscow, Russia. Department of Biotechnology, Mendeleev University of Chemical Technology of Russia, 125480 Moscow, Russia. A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory 1-40, 119991 Moscow, Russia" |
| DOI: | 10.3390/microorganisms10081512 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) emitted by bacteria play an important role in the interaction between microorganisms and other organisms. They can inhibit the growth of phytopathogenic microorganisms, modulate plant growth, and serve as infochemicals. Here, we investigated the effects of ketones, alcohols, and terpenes on the colony biofilms of plant pathogenic Agrobacterium tumefaciens strains and swimming motility, which can play an important role in the formation of biofilms. It was shown that 2-octanone had the greatest inhibitory effect on biofilm formation, acting in a small amount (38.7 g/m(3)). Ketone 2-butanone and unsaturated ketone beta-ionone reduced the formation of biofilms at higher doses (145.2-580.6 and 387.1-1548.3 g/m(3), respectively, up to 2.5-5 times). Isoamyl alcohol and 2-phenylethanol decreased the formation of biofilms at doses of 88.7 and 122.9 g/m(3) by 1.7 and 5 times, respectively, with an increased effect at 177.4 and 245.9 g/m(3), respectively. The agrobacteria cells in mature biofilms were more resistant to the action of ketones and alcohols. These VOCs also suppressed the swimming motility of agrobacteria; the radius of swimming zones decreased ~from 2 to 5 times. Terpenes (-)-limonene and (+)-alpha-pinene had no significant influence on the colony biofilms and swimming motility at the doses used. The results obtained represent new information about the effect of VOCs on biofilms and the motility of bacteria" |
| Keywords: | Agrobacterium tumefaciens alcohols biofilms ketones swimming motility terpenes volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINESidorova, Daria E Skripka, Mariia I Khmel, Inessa A Koksharova, Olga A Plyuta, Vladimir A eng numero sign 121030200227-6/the state assignment of the NRC 'Kurchatov Institute' - IMG for 2021-2022/ Switzerland 2022/07/28 Microorganisms. 2022 Jul 26; 10(8):1512. doi: 10.3390/microorganisms10081512" |
