| Title: | Functional differentiation of three pheromone binding proteins in Orthaga achatina using mixed-type sex pheromones |
| Author(s): | Si YX; Guo JM; Liao H; Li Y; Ma Y; Zhu YW; Wei ZQ; Dong SL; Yan Q; |
| Address: | "Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, College of Plant Protection, Nanjing Agricultural University, 210095 Nanjing, China. Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, College of Plant Protection, Nanjing Agricultural University, 210095 Nanjing, China. Electronic address: yanqi@njau.edu.cn" |
| DOI: | 10.1016/j.pestbp.2022.105097 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Pheromone-binding proteins (PBPs) play important roles in perception of insect sex pheromones, functioning to recognize and transport pheromone components onto the olfactory receptors of the odorant sensing neurons. Orthaga achatina, a serious pest of camphor trees, uses a mixture of three Type I (Z11-16:OAc, Z11-16:OH and Z11-16:Ald) and one Type II (Z3,Z6,Z9,Z12,Z15-23:H) sex pheromone components in its sex communication, in which Z11-16:OAc is the major component and others are minor components. In this study, we for the first time demonstrated that the three PBPs differentiated in recognition among pheromone components in a moth using mixed-type sex pheromones. First, tissue expression study showed that all three PBPs of O. achatina were expressed only in antennae and highly male-biased, suggesting their involvement in perception of the sex pheromones. Second, the three PBPs were expressed in Escherichia coli and the binding affinities of PBPs to four sex pheromone components and some pheromone analogs were determined by the fluorescence competition binding assays. The results showed that OachPBP1 bound all four sex pheromone components with high binding affinity, while OachPBP2 had high or moderate binding affinity only to three Type I components, and OachPBP3 had high binding affinity only to three minor pheromone components. Furthermore, key amino acid residues that bind to sex pheromone components were identified in three PBPs by 3-D structure modeling and ligand molecular docking, predicting the interactions between PBPs and pheromone components. Our study provides a fundamental insight into the olfactory mechanism in moths that use mixed-type sex pheromones" |
| Keywords: | Animals Carrier Proteins Insect Proteins/metabolism Molecular Docking Simulation *Moths/metabolism Pheromones/metabolism *Sex Attractants/metabolism Ligand binding assay Mixed-type sex pheromone Molecular docking Orthaga achatina Pheromone binding protein; |
| Notes: | "MedlineSi, Yu-Xiao Guo, Jin-Meng Liao, Hui Li, Yu Ma, Yu Zhu, Yu-Wei Wei, Zhi-Qiang Dong, Shuang-Lin Yan, Qi eng 2022/06/18 Pestic Biochem Physiol. 2022 Jun; 184:105097. doi: 10.1016/j.pestbp.2022.105097. Epub 2022 Apr 12" |
