| Title: | Emissions of volatile odorous metabolites by Clostridium perfringens - in vitro study using two broth cultures |
| Author(s): | Sharma NK; Keerqin C; Wu SB; Choct M; Swick RA; |
| Address: | "School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia. Poultry Cooperative Research Centre, University of New England, Armidale, New South Wales 2351, Australia. School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia. Electronic address: rswick@une.edu.au" |
| ISSN/ISBN: | 1525-3171 (Electronic) 0032-5791 (Linking) |
| Abstract: | "An in vitro study was carried out to investigate the volatile odorous metabolites produced by necrotic enteritis inducing Clostridium perfringens (Cp) type A field strain (EHE-NE18) cultured in two broth media (thioglycollate broth, brain heart infusion broth) at three levels (0, 103 cfu/mL, 106 cfu/mL) using 2 x 3 factorial arrangement of treatments, with each replicated three times. The culture media with and without Cp was freshly prepared on the day of measurement and the culture headspace concentration of odorants was measured using selected ion-flow tube mass spectrometry (SIFT-MS). The results showed that media type and Cp level affected the concentration of a range of volatile odorous metabolites including sulfur compounds, alcohols, ketones, amines, and carboxylic acids. Thus, Cp contributes to the production of a wide range of odorous metabolites that can impart noxious smell during infection" |
| Keywords: | Animals Chickens Clostridium Infections/metabolism/microbiology/veterinary Clostridium perfringens/*physiology Culture Media/*analysis Mass Spectrometry Odorants/*analysis Poultry Diseases/metabolism/microbiology Volatile Organic Compounds/*analysis Clost; |
| Notes: | "MedlineSharma, Nishchal K Keerqin, Chuck Wu, Shu-Biao Choct, Mingan Swick, Robert A eng England 2017/06/28 Poult Sci. 2017 Sep 1; 96(9):3291-3297. doi: 10.3382/ps/pex129" |
