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J Vis Exp

Title:		In vivo imaging of Dauer-specific neuronal remodeling in C. elegans
Author(s):		Schroeder NE; Flatt KM;
Address:		"Department of Crop Sciences, University of Illinois Urbana-Champaign; nes@illinois.edu. Department of Crop Sciences, University of Illinois Urbana-Champaign"
Journal Title:		J Vis Exp
Year:		2014
Volume:		20140904
Issue:		91
Page Number:		e51834 -
DOI:		10.3791/51834
ISSN/ISBN:		1940-087X (Electronic) 1940-087X (Linking)
Abstract:		"The mechanisms controlling stress-induced phenotypic plasticity in animals are frequently complex and difficult to study in vivo. A classic example of stress-induced plasticity is the dauer stage of C. elegans. Dauers are an alternative developmental larval stage formed under conditions of low concentrations of bacterial food and high concentrations of a dauer pheromone. Dauers display extensive developmental and behavioral plasticity. For example, a set of four inner-labial quadrant (IL2Q) neurons undergo extensive reversible remodeling during dauer formation. Utilizing the well-known environmental pathways regulating dauer entry, a previously established method for the production of crude dauer pheromone from large-scale liquid nematode cultures is demonstrated. With this method, a concentration of 50,000 - 75,000 nematodes/ml of liquid culture is sufficient to produce a highly potent crude dauer pheromone. The crude pheromone potency is determined by a dose-response bioassay. Finally, the methods used for in vivo time-lapse imaging of the IL2Qs during dauer formation are described"
Keywords:		"Animals Caenorhabditis elegans Microscopy, Phase-Contrast/methods Models, Animal Neuroglia/cytology/physiology Neuronal Plasticity/drug effects/*physiology Neurons/drug effects/*physiology Pheromones/pharmacology/physiology Stress, Physiological/physiolog;"
Notes:		"MedlineSchroeder, Nathan E Flatt, Kristen M eng R01 DK059418/DK/NIDDK NIH HHS/ R01 GM111566/GM/NIGMS NIH HHS/ 5R01DK59418/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Video-Audio Media 2014/09/17 J Vis Exp. 2014 Sep 4; (91):e51834. doi: 10.3791/51834"