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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Investigation of cell culture volatilomes using solid phase micro extraction: Options and pitfalls exemplified with adenocarcinoma cell lines
Author(s):		Schallschmidt K; Becker R; Jung C; Rolff J; Fichtner I; Nehls I;
Address:		"Federal Institute for Materials Research and Testing (BAM), Berlin, Germany. Federal Institute for Materials Research and Testing (BAM), Berlin, Germany. Electronic address: roland.becker@bam.de. Experimental Pharmacology & Oncology Berlin-Buch GmbH, Berlin, Germany"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2015
Volume:		20151020
Issue:
Page Number:		158 - 166
DOI:		10.1016/j.jchromb.2015.10.004
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		Three strategies to sample volatile organic compounds (VOC) from lung cancer cell lines cultured in vitro were compared. Headspace solid phase microextraction was applied in situ to culture flasks and alternatively to subsamples of headspace gas or to nutrient solution subsamples followed by gas chromatography-mass spectrometry. The direct quantification of 55 VOC in the headspace of cell cultures was validated and is discussed with respect to reproducibility and system-related interferences. The role of the VOC background from culture media and usually employed polystyrene culture vessels is examined and was seen to invoke potentially misleading conclusions. The commercial A549 and two further adenocarcinoma cell lines displayed largely similar VOC profiles with distinct differences regarding certain individual substances. There is evidence for the inappropriateness of the standard cell culturing methods in the search for volatile cancer markers
Keywords:		"Adenocarcinoma/*metabolism Analysis of Variance Cell Line, Tumor Gas Chromatography-Mass Spectrometry Humans Male Middle Aged Reproducibility of Results Sensitivity and Specificity Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis;"
Notes:		"MedlineSchallschmidt, Kristin Becker, Roland Jung, Christian Rolff, Jana Fichtner, Iduna Nehls, Irene eng Netherlands 2015/11/10 J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 1; 1006:158-166. doi: 10.1016/j.jchromb.2015.10.004. Epub 2015 Oct 20"